Diffusion Beetroot Essay

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Effect of Temperature on the movement of pigment by diffusion through Cell Membranes Aim: To investigate the effect of temperature on the rate at which the anthocyanin pigment leaves beetroot tap root cells Hypothesis: Beetroot cells have membranes which contains a phospholipid bilayers. These help create a stable fluid mosaic and retain their contents. The fluidity of the mosaic increases with temperature and high temperature may result in the membrane becoming unstable. This would result in the anthocyanin pigment to leak from the cells. Experimental Hypothesis: An increase in the temperature of water, in which beetroot tap root cylinders are bathed in for one minute, from room temperature (20 oC) to 70 oC will cause an increase in the amount of anthocyanin pigment in the solution (as measured by the colorimeter). Variables: * Independent: Temperature of the water bathing the beetroot cylinder * Dependent: The absorption of blue light by the anthocyanin in the solution (as measured by the colorimeter) * Controlled: Same beetroot is used to take the cylinder cuts, Size and dimensions of the beetroot cylinders, Volume of Water in which the beetroot is bathed, Length of time the beetroot cylinder is bathed Materials: * 1 large sized beetroot * Core Borer * Beakers * Measuring Cylinder * Test Tube rack * Test Tubes * Distilled Water * Colorimeter * 4 Water Heaters * Knife * Ruler * Thermometer * Stopwatch Method: 1. Get one large beetroot and with the use of the core borer cut 6 beetroot cylinders from the beetroot. Make sure to use the same sized core borer when extracting the cylinders. 2. Cut the beetroot cylinders, with a knife, to exactly 3 cm. Use a ruler to measure 3 cm from the cylinder. 3. Then wash the beetroot cylinders with the use of water. 4. With the use of a

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