Diffusion and Osmosis in Sugar Solutions

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TITLE: Diffusion and Osmosis in sugar solutions PURPOSE: The purpose of this lab was to study the effects of osmosis and diffusion on sugar solutions of varying concentrations through a selectively permeable membrane. The experiment will show how molecules in a solution are able to move from an area of high concentration to an area of low concentration. With this, it will also show us how hypertonic and hypotonic solutions exist, as well as how cells try to get back into an isotonic state. In doing so, this lab will allow us to learn why and how diffusion and osmosis happens in all living things. HYPOTHESIS: I hypothesize that the more concentrated sugar solutions will cause a greater amount of osmosis to make each solution isotonic. MATERIALS AND METHODS: Materials: Scale or balance, 24” dialysis tubing, 4 transfer pipets, sugar, scissors, rubber bands, 4 coffee sups of same size, 250ml graduated cylinder, ruler, small sauce and & 3 clean containers (600mls or larger), Methods: 1. Four 6-inch pieces of dialysis tubing were cut and soaked in a coffee cup filled with tap water for 2 hours prior to your start time. While waiting, I prepared the three sugar solutions. A) Added 5 grams of sugar to the 250ml graduated cylinder and then added water up to the 250ml mark. Placed a small piece of plastic wrap over the top of the graduated cylinder and mixed the sugar with the water. Then poured the contents into a small saucepan over the stove. Filled the graduated cylinder up to the 250ml mark again and then poured the tap water into the saucepan as well. Heated the mixture on the stove and stirred until the sugar has dissolved. Ater this the solution was removed from the saucepan and poured into a container and labelled that container 1% sugar solution. B) Rinsed the saucepan and then added 100 grams of sugar to the 250ml graduated cylinder and then added water up to

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