Copper Toxicity Essay

638 Words3 Pages
As I have done the introduction to copper toxicity, here I’m going to explain what are the procedures need to be done to have a fair result to find out the amount of copper present in a given sample. In order to explain these procedures I would prefer to recall my practical 3 where I have done the series of dilutions of 0.1M Cupric Sulphate which I prepared in my practical 2. From the results now I’m going to explain to a person who doesn’t have a previous knowledge regarding the measurement of presence of Copper in a given sample. Materials and apparatus required: - Cupric sulphate (CUSO4.5H2O) - Electronic balance - Pipette (P200, P1000, P5000) - Spectrophotometer - Cuvette. - Test tube - Distilled water - Volumetric flask. - LP4 tubes First 25ml of 0.1M Cupric Sulphate was made where the amount required was found in the following way... n=CV n-moles C-concentration V- Volume n= 0.1 x 25 x 10-3 =2.5 x 10-3 moles Then n = w/m n-moles w-mass required m- molecular weight 2.5 x 10-3 = w/249.71 molecular weight of CUSO4.5H2O=249.71 W = 0.624g Then as usual the solution was made by using the measured Cupric sulphate with the addition of water up to the point of 25ml in the volumetric flask. Then from the 0.1M stock solution of Cupric Sulphate the series of dilution was done as in the following table. The below mentioned volume of cupric sulphate was mixed with the corresponding volume of water as the final volume would be 2ml. The volume of cupric sulphate required for each concentration was calculated in the following way. C1V1 = C2V2 C1-Beginning concentration V1- Volume required C2- Final concentration V2- Final volume One example is shown below for standard 2. C1V1 =C2V2 0.1M X V1 = 0.02 X
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