The column is now ready for the addition of the mixture to be separated. 2.) Separation of the Fluorene and 9-Fluorenone Mixture: Accurately weigh 0.1g of the 1:1 mixture of fluorene and 9-fluorenone mixture, place it in a small test tube, and dissolve it in 0.5mL of petroleum ether. Add a few drops of dichloromethane, just enough to effect solution. Transfer the solution to the top of the column with a Pasteur pipet.
Add 3.0 mL of 1.0 M Copper (II) nitrate to the test tube. Add 5.0 mL of sodium hydroxide to the test tube; mix the reaction with a stirring rod. Feel the bottom of the tube and write down any changes you notice. Place test tube into the heated beaker and heat the tube until no changes are happening. Write down everything you notice.
Now to begin, pour 50 mL of the sodium phosphate buffer solution with a pH of 6.84 into the 150 mL beaker. From here on out, the sodium phosphate buffer solution will be referred to as simply the buffer solution. Next, locate the indicator called bromothymol blue (0.04%) and add 20 drops to your 150 mL beaker. The solution should then appear green. Next, obtain a 5 mL serological pipet and thoroughly rinse it with the buffer solution, then discard the buffer solution into the 250 mL beaker.
Overall reaction of the acid-catalyzed dehydration of 2-methylcyclohexanol Figure 2. Reactions for the unsaturation tests Table of Reactants and Products: Table 1. Molecular weight, density, melting point, and boiling point for all reagents used in Experiment #7 – Dehydration of 2-Methylclyclohexanol, Tests for Unsaturation, and Gas Chromatography. Name: | MW (g/mol): | Density (g/mL): | MP (C): | BP (C): | MSDS: | 2-methylcyclohexanol | 114.19 | 0.93 | -38.00 | 165-168 | Irritant, flammable | 95% phosphoric acid | 97.994 | 1.69 | 42.35 | 158 | Corrosive, irritant | 1-methylcyclohexene | 96.17 | 0.81 | -120.4 | 110 | Irritant, flammable | 3-methylcyclohexene | 96.17 | 0.81 | -124.00 | 104 | Irritant, flammable | Procedure: Part One – Dehydration of 2-methylcyclohexanol A microdistillation apparatus was assembled by securing a microdistillation flask to a ring stand and submerging it in an empty heating mantle. A thermometer and Teflon adaptor were tightly sealed into the top of the flask with the thermometer bulb being below the side arm.
II. Procedure Use a 10-mL graduated cylinder to put 6mL of 6 M sodium hydroxide solution (NaOH) into a clean 500mL storage bottle. Use a 100mL graduated cylinder to add 345mL of distilled water to the plastic bottle. Place a lid on the bottle and mix by shaking repeatedly. Sit the bottle off to the side for later use.
The last two cations should be separated with the observations of the additional NH4OH added. With that all the cations are separated, a confirmation test was given to all the cations. 20 drops of each cation solution were placed in new centrifuge tubes. A Bunsen burner was set up with a sharp blue cone that was inside of the flame, then a Nichrome loop was dipped into HCl then dipped into one of the cation
In this experiment, the salicylic acid is insoluble in cold water and can be collected by vacuum filtration. Procedure As the lab, synthesis of salicylic acid, had done, micro-scale was used. To begin, 3.5mL of water was poured into a 10 mL round-bottomed flask. And 0.48 g of sodium hydroxide was added to the flask. After the solid was dissolved, 230mg of methyl salicylate was added using a graduated pipet to the NaOH solution.
Once it started boiling the temperature was taken and it was 102 degrees Celsius. After the first results were recorded and beaker C was cleaned out and put away, beaker B (5 grams of salt) was placed onto the hot plate. As beaker b started boiling the temperature was recorded at 100.8 degrees Celsius. The results were then written, the beaker was cleaned out, and then put away. The
Then wash the zinc in the boiling tube “R” by adding 5 mL of acidified water, swirling and then decanting the solution into the “p” boiling tube. Repeat it 2 more times. | The liquid in the boiling tube “R” first starts out clear, and then once we add the acetic acid and swirl, the solution turns a yellow color, to then a brown-red color. The boiling tube
Then wash the zinc in the boiling tube “R” by adding 5 mL of acidified water, swirling and then decanting the solution into the “p” boiling tube. Repeat it 2 more times. | The liquid in the boiling tube “R” first starts out clear, and then once we add the acetic acid and swirl, the solution turns a yellow color, to then a brown-red color. The boiling tube