Catalase Lab Essay

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Lab Report Catalase Enzyme Lab Catalase Enzyme Lab Background: Catalse is an enzyme normally found in many plants and animals. Its purpose is to defend off any toxic substances by destroying them. It is also use by some cells to destroy cellular waste. This lab will demonstrate the effect of temperature and pH on the action of this enzyme. The substrate of the enzyme will be hydrogen peroxide (H2O2). Catalase works by the following mechanism. 2H2O2 ( 2H2O + O2 What is the effect of temperature and pH on the activity of catalase? Aims: Demonstrate and observe the effect of surface area of the enzyme, temperature and pH on the activity of catalase Materials: • 1M. NaOH Hot Plate 10 ml graduated cylinder • 1M. HCl Ice distilled water • 3% H2O2 2 large beakers potato • 6 test tubes Forceps liver • Test tube rack 3 micropipettes mortar and pestle • 3 thermometer knife Stop watch • Test tube holder Stirring rod Part A – Normal Catalase Reaction Hypothesis: Crushed potato will have the highest rate of reaction Procedure: 1- Take two clean test tubes and mark them 1 and 2. 2- Place a piece of potato in tube 1. 3- Pour 2ml of hydrogen peroxide to tube 2, and determine temperature. 4- Pour content of tube 2 to tube 1, and observe. 5- Pour off the liquid in tube 1, leaving the piece of potato. 6- Add another 2 ml of hydrogen peroxide to tube 1, and observe. 7- Using the mortar and pestle, crush a piece of potato and place it in a clean test tube (3). 8- Pour 2ml of hydrogen peroxide into tube 3, and observe. Data Collection: Quantitative Table 1: Recyclability and surface area effects on enzymatic activity |Type of reaction |Uncrushed potato (control) (22 c) |Recycled potato (catalase) |Crushed Potato

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