What is the function of the iodine solution in the Gram stain? If it were omitted, how would staining results be affected? To fix the dye on the slide in order to form insoluble substance, and if it is ommitted then, when flushed with water and the alcohol decolorized, the bacteria will come off. 2. What is the purpose of the alcohol solution in the Gram stain?
Then weigh the crucible without the hydrate after heating. Record both masses. Next, add CoCl2 ∙6H2O and weigh the crucible. Now place the hydrate and crucible on the hot plate. Observe the color change while it is being heated.
palladium on carbon 13 of 22 4/16/12 9:15 PM StudyBlue Flashcard Printing of Lab Final 2211L UGA http://www.studyblue.com/servlet/printFlashcardDeck?deckId=... Which alkyl halide would react fastest in a nucleophilic substitution using silver nitrate in ethanol? 3-bromo3-methylpentane bc its sn1 Which alkyl halide would react fastest in a nucleophilic substitution using sodium iodide in acetone? 1-bromopentane bc its sn2 What is the name of the chemical reagent used as the source of Br2 in the preparation of diphenylacetylene? pyridinium hydrobromide perbromide type of stationary phase column length column temperature rate flow of the carrier gas List the 4 general factors that affect the separation obtained on a gas chromatograph What specific technique is used to collect/isolate your purified unknown compound at the end of the recrystallization experiment?
E.g. of antibiotic is Vancomycin. Inhibit nucleic acid metabolism: blocks the recycling of the lipid carrier molecule needed to transport the peptidoglycans in the bacteria. E.g. of antibiotic Bacitracin (Reference for all the answers to question 3 = and ) 4.
Name: Dillan Taylor 1.3.1.2 - Human Homeostasis Vocabulary: dehydration, heat stroke, homeostasis, hypothermia, involuntary, thermoregulation, voluntary Prior Knowledge Questions (Do these BEFORE using the Gizmo.) A thermostat is a device that regulates the temperature inside a building. 1. What does a thermostat do if it gets too cool? ____blows out hot air_________ 2.
Explain why it is important to denature sucrase before measuring product concentration. To insure we are measuring it under the conditions we are testing, not letting it continue to be active after removing it from the water bath. Results Table 1: Effect of pH on Sucrase Activity 1 2 3 average ph 3 0.166 0.167 0.194 0.176 ph 4 0.332 0.331 0.330 0.331 ph 5 0.634 0.676
The heat obtained when a known mass of alcohol or paraffin wax burns will be used to warm a measured volume of water. Enthalpy change can be defined as the amount of heat released or absorbed when a chemical reaction occurs at constant pressure. In an exothermic reaction, such as the case in this experiment, the following reaction takes place: H = H(products) - H(reactants) = negative (-ve) Specific heat is the amount of heat per unit mass required to raise the temperature by one degree Celsius. The relationship between heat and temperature change is usually expressed, where c is the specific heat. The relationship does not apply if a phase change is encountered, because heat added or removed during a phase change does not change the temperature.
Hydrate Lab The purpose of this lab is to analyze the percent water in a crystalline hydrate and to indentify the hydrate from a list of possible unknowns. The solid hydrate will be heated to remove the water, and the percent can be found by measuring the mass of the solid before and after heating. The hydrate will be indentified by comparing the percent water in the hydrate with the percent water calculated for the possible unknown. Before the lab there are pre-lab questions: 1. Describe the three general safety rules for working with a Bunsen burner.
Using the utility clamp, clamp the thermometer, attach the paper lid to the probe then move the probe close to the bottom but not touching the magnetic stirrer. Start the Logger Pro program on the computer. Open the file “13 Enthalpy” from the Advanced Chemistry with Vernier folder. Measure 50.0 mL of 2.0 M Hydrochloric acid, HCl, solution and pour into the styrofoam cup, make sure that the temperature probe is touching the solution. Measure 50.0 mL of 2.0 M Sodium Hydroxide, NaOH, solution but DO NOT ADD YET.
What factors did you need to control in your tests? -During these tests, temperature needed to be controlled. 6. If you’ve ever used hydrogen peroxide as an antiseptic to treat a cut or scrape, you know that it foams as soon as it touches and open wound. How can you account for this observation?