Determining the Anatomy of a Dicot Plant Using Iodine-Potassium-Iodide Stain and Sudan Dye on Jackfruit (Artocarpus Heterophyllus)

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DETERMINING THE ANATOMY OF A DICOT PLANT USING IODINE-POTASSIUM-IODIDE STAIN AND SUDAN DYE ON JACKFRUIT (ARTOCARPUS HETEROPHYLLUS) Annaliza B. Cainglet and Sarah Aurora W. Tabada ABSTRACT Flowering plants are generally classified into two groups namely; Monocots and Dicots. The main basis for the classification is the number of cotyledons. In addition, similarities in the structure and arrangement of their tissues also contribute to their grouping. Hence, this study aims to determine and differentiate the characteristics of dicot and monocot plants. Also, it aims to establish the effect of the Iodine-Potassium-Iodide (IKI) solution and Sudan stain on the plant specimens. In order to do this, histological and histochemical analyses were performed on a young Jackfruit plant with the aid of the Free Hand Sectioning technique. Results show that the stem of the Jackfruit has a concentrically arranged ground tissue with a defined cortex and pith. Its root exhibited vascular bundles in ranges of two to six with the pith not well-developed. Its leaves, on the other hand, showed a delineated palisade and spongy mesophyll. Also, it has a thicker cuticle in its upper epidermis compared to its lower epidermis. The IKI stained specimens turned dark blue in reaction to starch in parenchymatous tissues in the stem and root. In contrast, Sudan stained specimens turned reddish brown in reaction to lipids in the cuticle of the leaf. Therefore, the jackfruit is a Eudicot plant as proven by the results of the analyses. Furthermore, IKI solution is better used in plant specimens with parenchyma that stores starch while Sudan dye is good for specimens with lipid-containing structures. INTRODUCTION All living organisms are classified based on their characteristic features. Although the structures and components of living organisms uniquely differ from one another there is

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