Experiment 11 is composed of cold liver and 2 mL H2O2. We anticipated that the reaction rate should be very low because a low temperature means a low reaction rate. At low temperatures, particles move slower and therefore there is a lower amount of collisions between the enzymes and the substrates. Based on observations, the reaction rate was fairly high which came as a surprise for us, this is not what we had anticipated. The reaction rate was given a 3.
Abstract: In this lab, the purpose was to determine the reaction rate for the absorption (concentration) of the reaction. The components needed for the rate law in this lab are the k value as well as determining if the reaction is zero, first, or second order. The graphical method will be used to determine the order as well as the “K” value. The absorption of the green light increased over time as the reaction occurred. Background: A reaction rate is the decrease in concentration of a reactant or the increase in concentration of a product with time.
Effect of Venturicidin on Oxygen Evolution Venturicidin is a powerful inhibitor of the chloroplast ATP synthase, interacting with the CFo part of the enzyme and blocking proton passage through the CFoCF1 complex. How would venturicidin affect oxygen evolution in a suspension of well-illuminated chloroplasts? Would your answer change if the experiment were done in the presence of an uncoupling reagent such as 2,4-dinitrophenol (DNP)? Explain. Answer Oxygen evolution requires continuing passage of electrons through PSII.
What is the role of the citric acid cycle? Include the reactants and the products. Where does it occur? Each of the pyruvic acid molecules will be processed in two separate citric acid cycles. Here the three carbon pyruvic acid molecule is processed to produce ATP and carbon Dioxide molecules.
In all the separation was pretty successful in getting Rf values for the three fractions, and the two compounds. Between the benzil and benzoin compounds, it was found that benzoin is the most polar. Benzoin was the most polar because it traveled the least amount of distance on the TLC plate. Conclusion: The results of this lab was that three different Rf values were calculated for the three fractions, and to measure their polarity from most to least polar. Fractions 1,2, and 3 were all separated by column/flash chromatography and their purities were measured with the TLC plate.
Cellular Metabolism Case Study Part 1: 1. Given the autopsy report the massive cell death in the kidneys, lungs, thymus, and heart indicate that the damage occurred at the cellular level, the parts of cellular respiration that occur in the mitochondrion. More specifically, the poison directly affected the mitochondrion and in doing so it suggests that cellular respiration was disrupted. 2. The cellular process that would be impaired by arsenate would affect the cellular process of glycolysis which occurs in the cytosol of a cell.
The rate of osmosis is affected by variables, two of them being temperature and concentration. In this experiment, the class will divide into two sections: some groups will look at the change in osmosis due to temperature, and the other groups will look at the change due to concentration. In osmosis, water moves from high concentrations of water to low concentrations of water. The water will try to move from outside the bag to the inside in order to dilute the solute, which in our experiment, is sucrose. Phospholipid bilayers and protein molecules form a semi-permeable boundary around the cell.
• First, each antacid tablet is mixed with 40 mL of 0.1 M HCl—this acidic solution is the same stuff that is in stomach acid, and one antacid pill is nowhere near enough to neutralize all 40 mL of the acid. • So, to see how much extra help each antacid pill needs to neutralize 40 mL of 0.1 M HCL, you add 0.05 M NaOH drop-by-drop to back-titrate the solution until the pH is neutral. • What this means is that, the stronger the antacid tablet, the less NaOH it will take to help bring the acid to neutral. (In other words, the stronger antacid tablets counteract more of the original HCl, leaving the solution closer to neutral before the NaOH is added.) Here are your results: Maalox Tums Mylanta CVS brand Rennies Mass of one dose antacid 20.0 g 21.0 g 18.0 g 18.3 g 17.5 g mL NaOH used in back-titration 24.1 mL 22.4 mL 20.0 mL 19.9 mL 24.4 mL 1.
Fumaric is catalyzed by succinate dehydrogenase. This occurs when H+ are moving from succinate to the electron carrier and the coenzyme FAD which is reduced to FADH2 Hypothesis: Our hypothesis was that the tube with the highest substrate will have the highest rate of reaction because it would have a higher amount than the substrate and it would be unable to compete. Our null hypothesis was that the tube with the lowest substrate will have the highest reaction. Experimental design: In this experiment we had a different electron carrier instead of the FAD. We use the 2,6 Dichlorophenolindophenol (DPIP) as the electron carrier.
Experiment 14: Iodine Clock reaction Summary of principles: In this lab we will learn to measure the rate of a chemical reaction between persulfate ion and iodide ion. The order of the action will need to be determined in the order of the persulfate ion to the iodide ion. The method we will use is the Initial rate method. The activation of the reaction will be determined from the temperature dependence rates of the reaction. The lesson of the lab is to teach us about Chemical kinetics, speeds and arrangements of molecules in a reaction during the process it goes from reactants to products.