Separation of Unknown Organics by Flash Column Chromatography

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Subject: Separation of unknown organics by flash column chromatography Introduction Column chromatography is a process that is used as a purification technique. The purpose of this lab is to isolate desired compounds from a mixture. The goal of this experiment is to apply column chromatography to separate two compounds and analyze their purity. It will be accomplished by running the mixture to be analyzed through the top of the column tube and then find purities by a TLC plate after the mixture has been separated. Reactions: Results: Calculations: Discussion: The TLC plate had 5 circles, indicating the 5 compounds, when it was under the UV lamp. Evidence shows that fraction 1 was the least polar because it traveled the farthest up the TLC plate. Fraction 2 was slight more polar than fraction 1, but less polar then fraction 3. Fraction 3 was the most polar because I traveled the least amount of distance. In all the separation was pretty successful in getting Rf values for the three fractions, and the two compounds. Between the benzil and benzoin compounds, it was found that benzoin is the most polar. Benzoin was the most polar because it traveled the least amount of distance on the TLC plate. Conclusion: The results of this lab was that three different Rf values were calculated for the three fractions, and to measure their polarity from most to least polar. Fractions 1,2, and 3 were all separated by column/flash chromatography and their purities were measured with the TLC plate. One suggestion for future labs would be to use more accurate measuring tools. Post lab 1. So the solvent would not reflect the UV rays, therefore creating a dark spot on the luminescent TLC plate. This dark spot helps us measure how much the solvent moved on the TLC plate. 2. There would be a larger dot making it harder to correctly measure how much the

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