Aseptic Technique Lab Report

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Lab 3: Aseptic Technique and Culturing Microbes Task: A. Describe the difference between an obligate and facultative aerobe. The difference between the two is Obligate aerobe requires oxygen to be present in order to grow an organism. With Facultative aerobe the organism does not necessary need oxygen to survive. It can live and grown with or without oxygen. B. Discuss the temperature requirements of the organisms in this lab. The temperature requirement for this lab was 37 degrees Celsius , it was very important to keep the temp at this level for the bacteria(S. epidermidis and L. acidophilus) to grow. According to the Labpaq 37degrees Celsius is considered “average” temperature to grow bacteria (Mesophiles). (labpaq, year unknown)…show more content…
Discuss the basic forms of culture media. Nutrient medium and MRS Medium are the basic forms; both come in liquid and solid form. There are other forms of culture media(i.e. Complex, Enrichment, Selective, Chemically Defined and differential media) G. Explain what each form of culture media is used for. According to Labpaq “Nutrient medium is the standard growth medium used for culturing most microbes”. MRS culture medium contains polysorbate, acetate, magnesium, and manganese, which act as “special growth factors” for lactobacilli. (labpaq, unknown year) H. Discuss whether you saw growth in the tubes after 24 hours. For the S. epidermidis I did not see any growth (uninoculated) after 24hr. For the L. acidophilus I saw sediment after 24hr. I. Discuss whether you saw growth in the tubes after 48 hours. For the S. epidermidis I did not see any growth (uninoculated) after 48hr. For the L. acidophilus I saw sediment and Turbidity after 48hrs. J. Explain why you did or did not see growth. I’m unsure exactly why I did not see growth with the S. epidermidis culture. It is my belief that no growth occurred due to maybe an error in collection or other microbes were present. I believe I was able to see growth with the L. acidophilus culture due to the L. acidophilus being separated and it didn’t require swabbing to

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