Cell Respiration Lab Report

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Stephen Simpson Access Med 2014 Cell Respiration “Micro-organisms” Abstract Cellular respiration is the process of obtaining energy from food, glucose is oxidised which produces ATP. The experiment was carried out to identify which Carbohydrate molecule was the most efficient for the respiration process, if respiration was taking place and to compare the respiration rate of the micro-organism yeast when conditions are changed. Five test-tubes were labelled A to E, rezasurin dye was placed in each test tube, to which fresh yeast was added, a carbohydrate was then added to test tubes A-D, in A glucose was added, B lactose, C Sucrose, D Starch and in E distilled water was added as a control for the experiment. All five test tubes were placed in a water bath at 35°. The colour change was monitored over a period of 20 minutes and changes recorded every 2 minutes. The results obtained showed that a colour change occurred in test tubes A to D with no change in colour in test tube E, the colour change was due to the gain of hydrogen “reduction” by the rezasurin as a result of “oxidation” in the respiration process. These results confirm that simpler carbohydrate molecules are oxidised faster in respiration than more complex carbohydrate molecules, the more complex the sugar the longer respiration takes; confirming also that if there is no sugar then there is no respiration. Introduction The process of obtaining energy from food is cellular respiration (Regina Bailey, 2014); this process is key to the cell being able to carry out its function. Respiration has two main methods anaerobic- takes place in the absence of oxygen and aerobic respiration- takes place in the presence of oxygen. The main stages of respiration are Glycolysis, TCA cycle and Electron Transport Chain. Glycolysis occurs in the cytoplasm of a cell, this process does not require oxygen and begins

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