The Effects Of Catachol With Potato Extract

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Pg. 1 The Effects of Catachol with Potato Extract Abstract: The effects of enzymes and substrate concentration on catecholase activity for Ph were determined in this experiment. To get the results, we mixed potato extract with catachol and then exposed it to different Ph levels. The Hypothesis that was being tested was to see if more amounts of H2O, catechol and the potato extract were added, would the reaction rate go up. Also if we added higher amounts of extract will it produce high color intensity. The results of my experiment supported the hypothesis that the rate of change of the substrate would increase with increased amounts of enzyme, catecholase. We observe red that Tube 1, which had the highest absorbance level. All three tubes had potato extract in them, it was used as a measure of reaction, the greatest rate of change of catechol and oxygen to benoquinone was seen in tube 1. Tube 2 also seen an increase in the reaction and color intensity. Tube 3, the one with no catecholase had none or very little reaction. Introduction: Enzymes are macromolecules serving as a catalyst, or a chemical agent that changes the rate of a reaction without being consumed by the reaction. Catecholase has an optimum level of ph between 6-8. There are some exceptions like Pepsin, a Digestive Enzyme which has a Ph of 2, which is found in the stomach of humans. In contrast, Trypsin is a digestive enzyme that works in the small intestines where the environment is much less acidic. Its Ph optimum is Ph 8. Environmental conditions are important in enzymatic reactions because temputure, ph, and chemicals that influence enzymes can influence them. Enzymes are practically or completely denatured if the ph is changed from the optimum Ph. We first learned how to work the spectrophotometer, bye first zeroing it out with our blank. Our blank contained potato

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