Reflection Paper

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Simge ENGELKIRAN Title of presentation: The Effects of Naltrexone on Spatial Memory after Restraint Stress in C578L/6J Mice: A Mechanism for Opiate Receptors and Stress Presenter: John Kummer The researchers hypothesis was being tested is that C57BL/6J mice that experience immobilization stress and are injected with naltrexone HCI will spend more time in the target zone of the Barnes Maze on the probe trial day than the C57BL/6J mice that experience immobilization stress and are injected with saline. The method for this experiment was using C57BL/6J mice at 3 weeks of age were split into IV groups, immobilization stress+ saline for six experimental rats and immobilization stress + 1 mg/kg naltrexone for another six experimental rats. The experimenter spend one week of habituation to food and cage was given. One day of habituation to injections and the Barnes Maze was given. And they spent another five days, over two weeks; the mice were given their IV on experimental days. During the experimental days, the mice were: firstly injected with the IV and allowed thirty minutes for the IV to become active. Secondly, pit into restraints for one half hour, and thirdly removed from the restraints. Finally, one half hour later, placed in the Barnes Maze for testing. After those five experimental days, the mice were given a non- stressed probe trial in which the escape box was removed from the Barners Maze, and the maze was split up into quadrants. The amount of time the mice spent in the home quadrant during the one minute trial was timed. The researcher used three different types of graphs and two different types of tests. He used t-test to compare how much time each mouse spent in the target quadrant zone over one minute in the Barnes Maze, and his results were no significant. The other test was one way ANOVA, the aim of this test was to see the number of times the
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