Mitochondria Tell Me Lab Report

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What My Mitochondria Tells You About ME. Abstract Mitochondrial DNA is a key to understanding genetic science. This lab breaks down what is a mitochondrion and its key uses. In this lab I prepared my mitochondrial DNA for sequencing. It was sent to Dolan Lab where I would be able to compare my DNA to that of my class mates and many others. Introduction Mitochondria are organelles located inside in the cell. They are the power houses of the cell. This is because they produce much of the ATP (adenosine triphosphate), which is the energy, used inside the cell to carry out many functions. And it also has many other important roles in cells like controlling the cell cycle and cell growth, signaling, cellular differentiation and cell death. The mitochondrion is different from other organelles in the cell. It does carry its own circular DNA. They are able self-replicate. It is also free of a nuclear envelope, and the DNA is not packed into chromatin. Most of this DNA from the mitochondrion is inherited from the mother. This DNA is packed by…show more content…
(This will leave enough product if you intend to sequence the mt control region.) Expel any air from the tip before loading, and be careful not to push the tip of the pipet through the bottom of the sample well. Load 5 µl of the pBR322-BstNI size markers into one lane of gel. Electrophorese at 100 volts for 1 hour and 15 minutes. Adequate separation will have occurred when the cresol red dye front has moved at least 50 mm from the wells. Gel may be stained either with 1 µg/ml ethidium bromide for 10 minutes or 0.05% methylene blue (or proprietary) stain for 30 minutes, followed by 20-30 minutes destaining with water. * If adding Bromophenol Blue loading dye to sample: do not add the loading dye to the entire sample, leave 10µl of sample without loading dye if you will be sending sample in for

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