Macromolecules Lab Report

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Introduction & Hypothesis In this experiment we are trying to test which macromolecules are present in baby formula. We believe the baby formula would test out positive for reducing sugars, and proteins based on the nutritional facts for baby formula in the back of our lab manual Materials & Methods: 1. Labeled six test tubes RS+, RS-, S+, S-, P+, P- (Positive and Negative Controls for reducing sugar, starches and proteins respectively). 2. Added 2 mL of Glucose Solution to RS+, 2 mL of Starch Solution to S+, 2 mL of Egg Albumin solution to P+. 3. Added 2 mL of diH2O to RS-, S-, and P-. 4. Labeled three test tubes 1, 2, and 3, and added 2 mL of milk to each. 5. Added 5 mL of Benedict’s Reagent to RS+, RS-, and tube #1, and boiled the tubes for three minutes. 6. Added one dropper full of Iodine to S+, S-, and tube #2, and agitated the tubes. 7. Added 2 mL of Biuret’s Reagent to P+, P-, and tube #3, gently agitated the tubes and allowed to sit for two minutes. Results: Macromolecules Present in Milk | Test For | Coloration of Positive Control | Coloration of Negative Control | Milk (+ or -) | Red. Sugars | Reddish Brown | Blue | + | Starch | Bluish Black | Brownish | - | Proteins | Purple | Blue | + | Figure 1. Using detection reagents to determine the presence or absence of macromolecules in milk. Milk tested positive for both reducing sugars and proteins, but it tested negative for the presence of starch. Discussion: Our hypothesis stated that reducing sugar and proteins are present in baby formula, while starch is not. Our hypothesis was correct, both reducing sugar and protein came out positive while the starch remind negative. Even so, there was some color differences in our experiment compare to the actual color of our control group. The reducing sugar was more of a reddish orange than brown but we believe it was because

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