The body will use these enzymes to get rid of energy. The body could not get rid of fructose without these two enzymes (cliffnotes.com, n.d.). Substrate acted on by aldolase B: Aldolase B can use F-1, 6-BP and F1P as substrates. F1P is a derivative of fructose. It is produced in the liver by fructokinase (ghr.nlm.nih.gov, n.d.).
I will investigate and determine the effects of substrate concentration and pH on the initial rate of an enzyme-catalyzed reaction. Procedure: First I am gonna adjust the pH levels in five different test tubes, and then I am gonna add certain amount of substrate to each of those different test tubes and record different reactions in each of the test tubes. I am going to repeat this step four more times for each weight of substrate.
1) ABSTRACT The purpose of this experiment was to carry out the effects of enzyme concentration, temperature, pH level, and an inhibitor on a type of enzyme called Perixodase. The purpose of the experiment was also to understand the relationship between the change in absorbance with time and the enzyme reaction rate. To perform the experiment, the class was divided into groups. Each group took on one of the factors affecting the enzyme. Before beginning the actual experiment, we used the mixing tables that were given to us to set up the test tubes with the appropriate substances.
A cuvette block, four cuvettes, three 50 mL beakers, one 150 mL beaker, and one 5mL pipet were obtained. The phosphate buffers NaH₂PO₄ and Na₂HPO₄ were procured weighing .4060g and .4106g respectively. The phosphate buffers were then transported to the 150mL beaker. Next 50mL of distilled water was measured using a graduated cylinder and added to the 150mL beaker. Twenty drops of bromothymol blue was also added to the 150mL beaker.
Materials and Methods The experiment was started by setting up a Spec 20 spectrophotometer and collecting materials such as glassware, stirring rods, and thermometers to mix solutions containing Bromothymol Blue. Approximately .416 grams of Na2HPO4 and .409 grams of NaH2PO4 were dissolved with 50mL of distilled water in a 150mL breaker to create a buffer solution. Twenty drops of 0.04% Bromothymol Blue were added to the solution and the pH was measured to be exactly 7 using a Vernier probe. 5mL of solution was then distributed to three 100mL beakers using a volumetric pipette. Each beaker was filled with either 1mL of 1.0 M HCl, 1mL of 1.0 M NaOH, or 1mL of water with a pipette, in order to create three solutions that had varying pH levels.
It would be classified as a base, if the number given by the probe was higher than seven. It is considered an acid, if the number given by the probe was lower than seven. If the number was seven it would be classified as neutral. Water and acetic acid is what makes up Vinegar. It was important to know what vinegar was made up of to fine the percent composition when continuing on with the second investigation of this laboratory.
Metabolism GRT1 Task 4 Sara E Boyd WGU May 28, 2015 Enzymes are complex proteins that are used as a catalyst in a reaction. A catalyst is used to lower the activation energy needed to complete the reaction. It can be used to speed up the reaction. An enzyme must have a specific shape and have a activation site. Enzymes have specific characteristics.
Chelilim87 Lab Report Effects of Temperature PH and Cofactors on Enzyme Activity Introduction Enzymes are catalytic proteins they speed up chemical reactions without be changed up or altered permanently in the process. Various enzymes used for different methods, they act as catalyst by lowering the activation energy for the reaction. Enzymes have specific shapes in which they occur. Part of the conformation the active site of the enzyme, where the actual catalysis occurs in the cells. The specific molecule on which an enzyme functions is the substrate.
(2.) The enzyme with its substrate was exposed to three different pH levels. The pH levels that the enzyme with its substrate was exposed to are 3, 7, and 9. The rate of reaction with the exposure of each pH level was then measured. The dependant variable of this experiment will be the rate of reaction of the enzyme catecholase with its substrate catechol.
MAINTAINING A BALANCE 1.1 Identify the role of enzymes in metabolism, describe their chemical composition and use a simple model to describe their specificity on substrates. - Enzymes are a biological catalyst found in living things that helps control which chemical reactions are to take place. - Most enzymes are made of protein. [pic] 1.2 Identify the pH as a way of describing the acidity of a substance. - pH is a way of describing the acidity or alkalinity of a substance.