For every 20 drops of solution you will add 0.1g of zinc to the new test tube. Repeat steps 3 and four until the solution is clear. If there ever exists too little of the solution to get enough drops, add up to 1mL of distilled water to the solution. 4. Once the solution is clear, retrieve at least ten drops of the solution and place them in a new test tube.
Materials and Methods Part 1 For the cation elimination test first 10 drops of potassium, iron (III), zinc (II), copper (II), and cobalt (II) were added to 5 centrifuge tubes and the color was recorded. Then for the metal hydroxide test, 6 M NaOH was added drop wise till a precipitate was formed. Each solution except potassium formed a precipitate, so then 10 additional drops of NaOH were added to the remaining solutions. Tubes were cleaned with distilled water and 6 M HCL. Next was the ammonia test 10 drops of each metal solution were added to new centrifuge tubes and 15 M NH4OH was added until the solution changed color or a precipitate was formed.
Put exactly 5.0 mL of water in the 10.0 mL graduated cylinder. Record this volume in your data table (10.0 mL). Label the first pipet "Acid." To calibrate the pipet, fill it with LIU-2 water. Holding the pipet vertically, add 20 drops of water to the cylinder.
The fungal culture was grown in 100 ml PDB for 5 days. The culture was harvested and the mycelial mat was separated by filtration using Whatman No. 1 filter paper. Then mycelium was ground separately in pestle and mortar using liquid nitrogen. About 50 mg of the powdered mycelium was transferred into a microtube contained 500 µl of TES (100 mMTris, pH 8.0, 10 mM EDTA, 2% SDS).
Repeat steps 1-5 for trial 2. Variables and Control test: Independent Variable: Amount of each reactant poured into the test tubes Dependent Variable: Height of each solution Constant variables: Amount of time waiting for solution Data Table: Test tube # | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Trial 1 | 0.5 cm | 1.0 cm | 1.1 cm | 1.5 cm | 1.5 cm | 1.5 cm | 1.3 cm | 1.5 cm | Trial 2 | 0.5 cm | 0.7 cm | 1.0 cm | 1.0 cm | 1.3 cm | 1.7 cm | 1.9 cm | 1.5 cm | Observation/Analysis: Solution turns yellow when a separate product is added, solid of the solution leaks down to the bottom after 20 minutes. Conclusion: The group tried to find the excess or limiting for the reaction between KI and Pb. In the end the hypothesis was supported by the data. We found that little amounts of each product led to a greater height of solids.
The dependent variable was the average time spent on the shape drawn within sixty seconds. The standardized variables were the type of pen and sharpie used, the size of the shape (circle) the termites were placed in, and the maximum time (60 sec.) given for the termites to be observed. There were two levels of treatment, which were red ink pen and red ink sharpie. The sample size was 100 termites and there were 5 replications for each level of treatment.
To determine if hyphae grow at the same rate, graph the results of table 2 and compare the slopes of the 3 hyphae from the slide preparations. 11. To determine if mycalolide B affects fungal tip growth and nuclear position repeat all the steps except in step 2 need to add mycalolide B and let it incubate for 10 minutes. Make sure to label the slides with the
The liquid of homogenate was filtered into a beaker through Miracloth (2 layers cloth) to remove large plant components and 1 ml of the filtrate was transferred to a conical tube. 8.4 g of ammonium sulfate was slowly added to the 40 ml of the filtrate as it was stirred on a stir plate for 15 min to achieve 37% saturation (210g/L of solution). The solution was then centrifuged at a speed of 9000 x g at 4oC for 15 min to sediment the proteins. The resultant supernatant 1 was transferred to a beaker with 1 ml transferred to a conical tube and the obtained pellet 1 was resuspended in 4 ml of distilled water and transferred into a dialysis bag to remove the salt. Then, 3.4 g of ammonium sulfate was slowly added to the supernatant 1 as it was stirred for 15 min to achieve 50% saturation (85g/L of solution).
Germination percentage and seedling growth (Plant high (cm), Shoot fresh weight (g) and Root weight (g)) of Zea mays (at 20 day age) were estimated. The experiment included 15 treatments with three replicates; all treatments were arranged in spilt plots design. The soil was used in the pots experiment can be described as follows:- sandy 89.9%, silt 7.1%, clay 3.1 %, pH 8.37, EC 0.33 dsm-1 , total nitrogen 0.003%, available phosphorus 8.31 ppm and organic matter