Fungal Tip Growth Lab Report

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Protocol: Hyphal Tip Growth The null hypothesis is that mycalolide B will not have an effect on fungal tip growth and nuclear position. The alternative hypothesis is that mycalolide B will have an effect on fungal tip growth and nuclear position. 1. Take off the tape and remove a slide from petri dish that has an agar cushion that is smooth, flat and no bubbles. 2. For the control, add 25 µl growth medium on hyphae with a Gilson pipette and yellow tip and cover with a slip over it. Take note of the time for each slide preparation. 3. Set up Kohler and observe the specimen on the slide with 10x objective lens then move to 40x objective lens, switch from BF to fluorescence. 4. Once in focus set up the ocular micrometer to be adjacent to the nucleus to…show more content…
After observing nuclei, switch back to BF and set up the ocular micrometer to be adjacent to fungal tip to determine fungal growth tip. 8. Using the ocular micrometer, measure and record the distance the tip grows in 5 minute intervals. Do this with 3 different hyphal tips on one slide prep and then switch to another slide. 9. Record observations for each hyphal tip in table such as the one below but do one for each hyphal tip and for two different slides. Calculate the speed of growth using s=distance/time. Table 2: Hyphal Tip with Growth Medium (Slide 1) Time(minutes) | Distance (um) | Speed (um/min) | | 1 | 2 | 3 | 1 | 2 | 3 | 5 min | | | | | | | 10 min | | | | | | | 15 min | | | | | | | 20 min | | | | | | | 10. To determine if hyphae grow at the same rate, graph the results of table 2 and compare the slopes of the 3 hyphae from the slide preparations. 11. To determine if mycalolide B affects fungal tip growth and nuclear position repeat all the steps except in step 2 need to add mycalolide B and let it incubate for 10 minutes. Make sure to label the slides with the

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