Daphnia Heart Rate Lab

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Daphnia Heart Rate LAB INTRODUCTION A Daphnia is a tiny crustacean (related to shrimp) that has a clear outside skeleton (carapace) and jointed legs. Like other arthropods, its heart is on its back. The environment challenges each living thing to respond. It includes the air, the water, heat and light, and the chemicals, which enter our bodies. Because Daphnia are ECTOTHERMS (cold-blooded), their body temperature changes with the surrounding environment. Since chemical reactions are speeded up in warmer temps, what would you predict the effect of temperature changes would be on their rate of metabolism (and heart rate)? Chemicals, which enter their bodies, can also change their heart rate by interfering with the chemicals that nerves use to transmit signals. Chemicals that speed up heart rate are known as stimulants, whereas chemicals that slow down the heart rate are known as depressants. HYPOTHESIS : What is the Dependent Variable measured: ______________________________________ What is the Independent Variable used: __________________________________________ Predict the effect of each situation/solution on the heartbeat: Make a Hypothesis for each Cold Water Hot Water 1% Ethanol Sugar Water MATERIALS • 4 Daphnia in culture liquid (per table) • Transfer pipette • a clean depression slide • Compound microscope • a small container for “used” Daphnia PROCEDURE – follow below or the modify to YOUR teacher’s instructions Keep the light for your microscope OFF as much as possible to avoid overheating your Daphnia! 1. Using a clean pipette, carefully transfer a Daphnia and ONE drop of liquid onto a slide. Keep the drop small so that the Daphnia can’t swim out of your field of view. 2. Place the slide under the microscope and focus on the Daphnia so that you can see the beating heart. REMEMBER: its heart is

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