* Then you will have to put the test tube in a water bath and leave it until the contents reach the same temperature as the water bath. * Then you will have to take the thermometer from the test tube and put a glass rod into it instead. * After this you will have to use a 2cm syringe to measure out 1cm of lipase to the beaker in the water for the temperature you are investigating. * Then add the lipase to the test
Effect of Caffeine on Daphnia Heart rate 24/09/2012 Core Biology Practical (1) Introduction: Caffeine is the drug taken most globally, and can cause health problems such as raised blood pressure. Since we are looking at the heart, we decided to test the effects exposing daphnia to caffeine would have on the daphnia’s heart. Daphnia’s are small water-fleas with transparent body therefore the heart can be visibly seen even without a microscope making the experiment easy to be carried out. Aim: In this investigation, I aim to determine the effect of caffeine on daphnia heart rate Hypothesis: An exposure to higher concentrations of caffeine will cause a distinct increase in the heart rate of daphnia. Null Hypothesis: An exposure to higher concentrations of caffeine will have no effect on the heart rate of daphnia.
Experiment 1: Extraction of Caffeine from Tea The primary objective of the experiment is to separate the caffeine from tea leaves. The mixture of tea and the calcium carbonate is separated from tea leaves by vacuum filtration. The solvent used in extraction of caffeine is dichloromethane. Anhydrous magnesium sulfate is added to the dichloromethane solution to dehydrate the mixture. The dichloromethane is then separated from the mixture in the rotary evaporator.
Then the filtrate goes through the descending loop of Henle, which is permeable to water (water retained back), and then through the ascending loop of Henle, which is permeable to salt (salt is retained back). Then the filtrate goes to the distal tubule, which removes the wastes that were not initially filtered out of the Bowman’s capsule (hydrogen ions). From the distal tubule, it travels to the collecting duct (now called urine), which prepares the urine
5. The burette was then refilled with the NaOH and the meniscus was adjusted so that it sat at 0.0mL 6. The pipette was filled with the acetic acid and then drained once again to ensure the pipette wasn’t contaminated as otherwise the obtained results could have been incorrect. 7. The pipette was then filled with 25.0mL’s of acetic acid and then drained into a conical flask, to speed up the process both of the conical flasks were filled with 25.0mL of the acetic acid.
It will be enough and you eat every day radish when you have a sinus infection. There are several recipes that all can use horseradish for sinusitis, and most are used as follows: in a liter of boiling water grate fresh horseradish root, and then inhale, ie. inhale the vapor, with a towel over his head. Horseradish will clear your sinuses, alleviate inflammation and uncork nose. Inhalation of the sinuses Inhalation is the good old proven way to cure sinusitis.
We recommend that you mix your own potting mix using a recipe like this: 1 part sphagnum peat moss, 1 part perlite, 1 part vermiculite. Many growers add a small amount of dolomite lime to control the pH. The potting mix should be pre-moistened before potting your violet, or the wicking process may not function properly. The wick that you use should be 6 to 8 inches long, and must be a man-made fiber such as acrylic, rayon, or nylon yarn or cording. The wick needs to be wet when it is used, and I find that soaking it in a little soapy water helps to break down the water resistance that some of the materials seem to have.
Effect of Temperature on the movement of pigment by diffusion through Cell Membranes Aim: To investigate the effect of temperature on the rate at which the anthocyanin pigment leaves beetroot tap root cells Hypothesis: Beetroot cells have membranes which contains a phospholipid bilayers. These help create a stable fluid mosaic and retain their contents. The fluidity of the mosaic increases with temperature and high temperature may result in the membrane becoming unstable. This would result in the anthocyanin pigment to leak from the cells. Experimental Hypothesis: An increase in the temperature of water, in which beetroot tap root cylinders are bathed in for one minute, from room temperature (20 oC) to 70 oC will cause an increase in the amount of anthocyanin pigment in the solution (as measured by the colorimeter).
The third test will utilize thin layer chromatography to evaluate the purity of the aspirin as well as testing for the presence of leftover salicylic acid or other by products of the reactions. Experimental: Week 1: For the synthesis of the aspirin, 250 mL of water was boiled. 1.5 g. of salicylic acid were poured on a test tube. Then, 3.5 mL of acetic anhydride and four drops of 85% phosphoric acid were added. A cotton ball was placed to prevent vapor escape.
The watch glass was removed with the beaker tongs. Using a rubber bulb and a stirring rod to stir the solution continuously, 15.00mL of .25M BaCl2 solution was added to the solution in the beaker. The watch glass is replaced and the solution is keep hot but not boiling for 15 minutes. The precipitate was allowed to settle. When the liquid above the precipitate was clear, the solution was tested for completeness of precipitation when a few drops of BaCl2 solution were added from a pipette.