The Role of Biological Buffers in Livinsg Organism

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NAME: JESSICA JAY BIO 211: CELL BIOLOGY DATE: 23 OCTOBER 2013 PRACTICAL 7: BIOLOGICAL BUFFERS TITLE: THE ROLE OF BIOLOGICAL BUFFERS IN LIVINSG ORGANISM INTRODUCTION A buffer is a mixture of molecules that release or bind H+ in order to maintain a relatively constant pH. Most buffers consist of a weak acid (which releases H+) and a weak base (which binds H+.) If additional base is added to a buffered solution, the acid part of the buffer releases H+ ions which combine with the extra OH-ions to form water. Therefore, the solution does not become as basic as it would have without the buffer. On the other hand, if additional acid is added to a buffered solution, the base component of the buffer combines with the H+ions that are released by the acid. This reduces the concentration of free H+ ions in the solution, so the solution doesn’t become as acidic as it would have if the buffer had not been present. (Schultz, 2006) A buffer is a substance that minimizes change in the acidity of a solution when an acid or base is added to the solution and it is used to make solutions of known pH. In living organisms, a buffer is used to keep enzymatic reactions of cells constant. Examples of buffering systems in living organisms are: the cytoplasm of plant cells and blood circulation in human beings. pH = pKa + log (⦋conjugate base⦌)/(⦋acid⦌) The above equations helps in relating pH and pKa, so as to know the suitable buffers as according to their pH. The pH scale was developed to measure how acidic or basic (alkaline) a solution is. (Dykstra & Reuss, 2003) Many chemical reactions in living cells involve exchanges of hydrogen ions. Because changes in acidity can affect both the structure and chemical reactivity of cellular molecules, cells must constantly maintain an acid-base balance. The aim of the experiment is to study the buffering effect in the number

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