The Effects of Different Enzymes on Apple Juice Production

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The Effects of Different Enzymes on Apple Juice Production By: Lars Schoppmann 12/5/14 Period 6 Mrs. Cushing Lab Partners: Matt Thompson and Chris Butler Introduction: The question of this experiment is " How do different enzymes affect the production of applesauce ?" The enzymes which gets tested are pectinase and cellulase. Cellulase is an enzym that is used to break down cellulose, wich reinforces the cell walls. Pectinase breaks down pectin , which breaks down cell walls of plant cells. The experiment hypothesis " If three drops of pectinase are stirred into applesauce and used to produce apple juice then it will produce more than water or cellulase because the fruit cells walls gets breaken down and gets turned into apple juice". The independent variable is the type of enzyme which gets mixed into the applesauce. The dependent variable is the amount of apple sauce which bgets created. A few controll variables are to use the same amount of applesauce and enzymes and to stirring the cup with the same amount everytime Materials: • Apple sauce • Pectinase enzyme • Cellulase enzyme • Funnels • Filter • Paper towels • Droppers (one for each enzyme) • Stirring rods • Graduated cylinder • Access to water • Beakers • timer Safety: • Put on safety glasses, or safety goggles. • Tie back any long hair that is hanging down. • Do not eat or taste any of the materials that are being used within the experiment. • Wear closed toed shoes at all times during the lab. • Do not wear any loose clothing or jewelry Procedure: 1. Gather all material 2. pour the applesauce in three cups with the same amount 3. add three drops of both enzymes and water in for each one material in one cup 4. Get timer ready and stir 3 minutes 5. apple juice from each cup into 3 seperate cylinders using rubbers and

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