Fruity Dna Extraction

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Fruity DNA Extraction Introduction Purpose: To see if changing the fruit will change how much DNA will show. Hypothesis: If there is a change in the fruit that the DNA will be extracted then there is no reason for the amount of DNA visibility shown to differ because we are not adding much difference in the procedure just changing the fruit that we are using from a strawberry to an orange and they are both fruits just different kinds. Background Information: One important characteristic of living is that their cells contain DNA. It doesn’t matter if you are a human, dog, bacteria, mushroom, or strawberry- all living cells contain DNA. In this lab I will extract the DNA from a strawberry and an orange for the extraction to analyze its effect. Experimental Design Variables: MV: Fruit in which DNA will be extracted RV: DNA visibility C: Time mashed, amount of detergent added, amount of pineapple juice added, amount of filtrate added in the test tube, amount of ethanol added Materials: Heavy Duty Zip lock Strawberry Orange 60 ml detergent-salt solution 2 ml pineapple juice (natural enzyme) 2 filter (gauze) 2 funnel 2 test tube 2 beakers 2 rubber bands 2 graduated cylinders 2 glass stirring rods Ice cold ethanol Procedure: Place 1 large strawberry into a zip lock bag and crush for about 1-2 minutes Add 30 ml of the soap detergent solution into the bag Crush and mash again until the solution is smooth Add 1 ml of pineapple juice to the bag Mix gently for 1 minute—just get the pineapple juice (containing natural enzymes) evenly distributed. Gently pour the solution through filter (gauze) into a beaker Funnel 6 ml of the filtrate (filtered liquid) in the test tube Obtain 6 ml of ethanol from the teachers lab station Carefully pour the ethanol

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