The Effect of Caffeine on the Heart Rate of Daphnia

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Activity 1.23 The effect of caffeine on heart rate of Daphnia Aim and Introduction Daphnia (also known as water fleas) are small aquatic crustaceans commonly found in fresh water ponds and lakes. Daphnia are primary consumers (they feed on algae) and are important prey organisms for many fish and other animals. The most obvious effect of caffeine in humans is that it raises the heart rate and makes us feel more energetic. As a matter of the fact, caffeine is the most popular drug in the world. In humans, nerve cells synapse with the pacemaker (SAN) region of the heart in the right atrium, which then initiates the heart beat/cardiac cycle. Caffeine is a chemical stimulant and may act directly on this pacemaker region, mimicking the stimulatory neurotransmitters, thus causing an increase in contractions/systole and therefore heart rate. There may be a similar effect of caffeine on the nervous system occurring in other animals e.g. crustaceans like Daphnia. I predict that as the caffeine concentration increases the heart rate of the Daphnia will increase. Method The independent variable for the experiment is the concentration of the caffeine that’s given to the daphnia. The dependent variable is the beat rate of the daphnia. There are other variables the needs to be controlled such as the temprature, size of daphnia, gender of daphnia, the amount of time we wait till we start counting the heart beat. Reasons for our method: Only putting the microscope lamp on when counting the heart rate of the Daphnia because this might alter the temprature and if the temperature goes up as may the heart rate and this is not facor we are investigating. That pond water is used as the daphnia are used to living there and we to keep as many features from their normal life as we can, so the results are more accurate. A cavity slide is used to help keep the daphnia in a small

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