The Effectivebess of Lipase on Temperature

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The effectiveness of lipase on temperature Abstract Enzymes are biological catalyst that speeds up the rate of reaction. Different enzymes work best at different temperatures, which is also called an optimum temperature. Different enzymes also have different functions. Lipases are enzymes, needed to break down lipids (fats and oils) into the products - glycerol and fatty acids. They work best at room temperature around 36-40 C. Introduction For this experiment, I will be looking at how the change in temperature affects the rate of reaction. If the temperature is too high or too low in the water bath the enzymes active site will denature. The best temperature is 37 degrees because this is the natural body temperature which is also called the optimum temperature so the reaction will take place faster. Method * Firstly you have to label a test tube with the temperature that you will be investigating. * Secondly you have to add five drops of phenolphthalein the test tube which you are using. * Then measure out 5 cm of milk using a syringe or a measuring cylinder, and then add this to the test tube. * After this measure out 7 cm of sodium carbonate solution using a different measuring cylinder or a syringe and then add this to the test tube, which will make the solution turn pink. * Next you will have to place a thermometer in the test tube, but you will have to be careful as the experiment could fall over. * Then you will have to put the test tube in a water bath and leave it until the contents reach the same temperature as the water bath. * Then you will have to take the thermometer from the test tube and put a glass rod into it instead. * After this you will have to use a 2cm syringe to measure out 1cm of lipase to the beaker in the water for the temperature you are investigating. * Then add the lipase to the test

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