Question doesn't relate to hypothesis - fix! AP Lab – Cellular Respiration Question: How does temperature affect the rate of cellular respiration in germinating and dry peas? Hypothesis: If both germinating and dry peas are placed in a respirometer and submerged in room temperature water and water with a temperature of 10 °C, then the respirometer with the germinating peas will prove a greater rate of cellular respiration than that of the dry peas in both temperatures of water. Procedure: See lab handout Data: See attached Analysis Questions #1-10 1. Conditions that must remain constant in this experiment include the temperature of the water baths and the volume of peas and beads in the respirometers.
Procedure: Refer to Chemistry Lab Manual pp. 33-35, 37. Results: The density of the yeast-sugar solution on day 1 was 1.05g/mL. On day 2, the density of the solution had dropped down to 1.00g/mL. We found the mass of our pure alcohol to be 10.23g.
(This will leave enough product if you intend to sequence the mt control region.) Expel any air from the tip before loading, and be careful not to push the tip of the pipet through the bottom of the sample well. Load 5 µl of the pBR322-BstNI size markers into one lane of gel. Electrophorese at 100 volts for 1 hour and 15 minutes. Adequate separation will have occurred when the cresol red dye front has moved at least 50 mm from the wells.
Put on a safety goggles. Use a thermometer to measure the initial temperature of the water in the soft drink can. Measure the initial mass on a mass scale. Place the fuel under the soft drink can (If the fuel is half a cashew nut or cheetos stick it to a wine cork using a pin, then wrap the wine cork with a sheet of aluminum foil). Set the stopwatch to 1 minute.
Hypothesis I predict that if the concentration of Hydrochloric acid is increased by 20%, the rate of reaction will also increase by 20%. This is because more molecules of Hydrochloric acid will be present, meaning there will be more molecules to react with. For particles to react, they must collide with one another, but for a successful collision, there must be enough energy for them to react. This is known as activation energy. Activation energy is defined as “the energy that an atomic system must acquire before a process (such as an emission or reaction) can occur”.
See Table 3: Effect of Sucrose Concentration on Sucrase Activity See Graph: Effect of Sucrose Concentration on Sucrase Activity 4. Was sucrase activity higher at 25 ºC or 55 ºC? 5. State how sucrase activity changes with increasing sucrose concentration. Sucrase activity steadily increases with increasing sucrose concentration until a plateau is reached.
Measure 50.0 mL of 2.0 M Sodium Hydroxide, NaOH, solution but DO NOT ADD YET. Click the Collect button to get the initial temperature of the HCl solution. After the first four readings have been recorded at the same temperature, add the NaOH solution. Make sure you have lowered the paper lid to keep from liquid coming out of the cup. Turn the Stirrer on, at a slow to medium speed.
Experiment 2 Time | Observations | 5 minutes | Bubbled like sugar | Once salt water was added | Turned soapy white and thick | Equation: METHOD 1) Put 2 cm3 of castor oil into a 250 cm3 beaker and add 10 cm3 of 5mol.dm-3 sodium hydroxide from a measuring cylinder. (Take care when measuring this out and adding it to the beaker to avoid any drips and spills.) 2) Heat the mixture gently over a tripod and gauze, STIRRING CONSTANTLY with a glass rod (otherwise the mixture erupts). Allow to simmer (boil gently) for 5 minutes. Steady the beaker using
Materials> Hydrochloric acid HCl(l)> Sodium triosulphate Na2S2O3(l)> 250 ml3 beaker> 50 ml3 conical flask> 2 x 25 ml3 measuring cylinder> Thermometer> Stop clock> Tea kettle> Blank card> Pen Variables Reaction time is the dependent variable, as it depends on the temperature of the solution. The temperature of the solution is the independent variable, as it does not depend in anything. This can also be concluded from the fact that it is the temperature that is changed in this experiment. The concentration and the amount of the reactants used are the controlled variables, as they have to stay constant throughout the experiment. Method of data collection It is important to carry out this experiment in a safe way, due to the fact that sodium thiosulphate and hydrochloric acid are being used.
Hypothesis : The rate of enzymatic reaction increases with the increase in substrate concentration until it reaches a maximum rate. An increase in substrate concentration means more substrate molecules are available, it means that there are more chances of collision between the substrate molecules and enzyme molecules for a catalytic reaction to take place. As more substrate molecules fill the active sites, more products are formed per unit time. At a certain substrate concentration, the rate of reaction will not increase further and become constant. The reaction is at a maximum rate.