Cytoplasm is located within the cell membrane and all cell types and contains all organelles and cell parts. Majority of all the important activities take place in the cytoplasm as cytoplasm consist of enzymes which are responsible for breaking down waste and also aid in metabolic activity. Nucleus: The nucleus is the control system of the cell. The nucleus contains the DNA of the cell. The function of the cell is to maintain the integrity of these genes and to control the activities of the cell by regulating the gene expression.
Each genome contains the information needed to maintain and create the organism. The process of genetic engineering involves extracting of a small piece of cellular DNA, called a plasmid, from the bacteria if organism involved in the manipulation. A very small section of the circular plasmid is then cut out by the restriction enzymes which act as molecular scissors. The gene from the organism being modified is then inserted into this space and the plasmid is therefore modified. The genetically modified plasmid is now inserted and introduces into a new organism which starts divides rapidly.
-After the gene is amplified a suitable vector is selected for use. A vector is a self-replicating DNA molecule used to transmit a gene from one organism to another. All vectors must contain the following characteristics: 1. Able to replicate host organism. 2.
7. The figure to the right shows that injection of M-phase cytoplasm into an oocyte triggers the cell to enter mitosis. What would be the expected effect on cell division if cytoplasm from a G2-phase cell were injected into an M-phase cell? A. DNA replication would begin again. B. activation of all cyclin-CDK complex in the cell.
Enzymes called topoisomerases produce breaks in the DNA molecules and then reconnect the strands, relieving strain and effectively preventing tangling and knotting during replication. DNA polymerase adds new nucleotides to a growing strand of DNA. Because DNA polymerase must adhere to an existing template, an RNA primer is first created at the site of replication. The RNA primer is synthesized by primase, an enzyme that is able to start a new strand of RNA opposite a DNA strand. After a few nucleotides have been added, the primase is displaced by DNA polymerase, which can then add subunits to the 3’ end of the short RNA primer.
This energy is then in turn used by the cell to carry out various functions. Nucleus- The main function of the cell nucleus is to control gene expression and mediate the replication of DNA during the cell cycle. Nucleolus- This takes up around 25% of the volume of the nucleus. This structure is made up of proteins and ribonucleic acids (RNA). Its main function is to rewrite ribosomal RNA (rRNA) and combine it with proteins.
During transcription, RNA polymerase makes a copy of a gene from the DNA to mRNA as needed. This process is similar in eukaryotes and prokaryotes. One notable difference, however, is that prokaryotic RNA polymerase associates with mRNA-processing enzymes during transcription so that processing can proceed quickly after the start of transcription. The short-lived, unprocessed or partially processed, product is termed pre-mRNA; once completely processed, it is termed mature mRNA. [edit] Eukaryotic pre-mRNA processingMain article: Post-transcriptional modification Processing of mRNA differs greatly among eukaryotes, bacteria, and archea.
The nucleus constitutes most of the genetic material of the cell - the DNA. It controls the heredity characteristics of an organism.It is responsible for protein synthesis, cell division, growth and differentiation. Cytoplasm: the cytoplasm is the fluid substance that fills the space between the cell membrane and the cellular organelles.The enzymes in the cytoplasm metabolize the macromolecules into small parts, so that it can be easily available for the other cellular organelles like mitochondria. It also transports the products of cellular respiration. Mitochondria: the mitochondria are rod shaped structures.
Proteins are formed through condensation reactions which bond amino acids together with peptide bonds in a particular sequence and the type of protein that is created is defined by the unique sequence of the amino acids. DNA and RNA are nucleic acids that are formed in the nucleotides and are both involved in the process of protein synthesis. Deoxyribonucleic acid, more commonly known as DNA, is located within the nucleus of the cell and contains the entire genetic code for an organism within its structure. DNA has two very important functions which are: to convey information from one generation of cells to the next by the process of DNA replication and to provide the information for the synthesis of proteins necessary for cellular function. Basically, DNA controls protein synthesis.
There has to be primers to start the synthesis at the 3’ end of the new strands. The RNA primers are later replaced with DNA. Leading & Lagging Strands DNA splits into 2 strands. The continuous strand (the leading strand), and the discontinuous strand (the lagging strand) that grows away from the replication fork. Death Cap Mushroom Transcription and Translation: mRNA is necessary to direct synthesis (transcription) of the polypeptides.