Opium Poppy

980 Words4 Pages
Forty seven genetic stocks of Opium poppy were screened for high seed yield and alkaloid contents. The estimate of heritability in the broad sense (h2BS%) was high for morphine content % (99.9) followed by the papaverine content % (99.85). The lowest h2BS% was recorded in days to 50% flowering (4.22%) and genetic advance (GA) for thebaine content % followed by papaverine. Genotypic (rg) and phenotypic (rp) correlation coefficient amongst codeine and thebaine content were highly significant and positively associated with morphine content followed by narcotine content. Papaverine content exhibited highest PCV (%) and GCV (%). The path coefficient revealed that the highest direct contribution to seed yield was made by poppy straw yield (0.8730)…show more content…
The solution was pooled, concentrated and redissolved in 1.0 ml methanol. The samples were centrifuged at 10,000 rpm before HPTLC analysis. The referred chemical is Morphine, Codeine, Thebaine, Papaverine and Narcotine were provided from Govt. Opium & Alkaloid Works, Neemuch, India. The purity of each opiate was ~98% by HPLC peak and normalization method. The stock solution of each estimated compound was prepared separately. The quantification was performed using linear regression curve. Validation: TLC-densitometric procedure was applied to quantify the five major opium alkaloids morphine, codeine, thebaine, papaverine and narcotine (Gupta and Verma, 1996). Toluene-acetone-methanol-ammonia (40:40:6:2) v/v was used as a mobile phase. Silica gel plates 60 F254 (Merck, Darmstadt, Germany) were scanned at 540nm after derivetization using Dragendorff reagent. 2.5 Statistical Analysis: Statistical analysis were done on the pooled data of the two years using the Statistical Software 4.0 version, available in the Division of Genetics and Plant Breeding of institute, based on the standard methods (Panse and Sukhatme, 1967; Dewey & Lu 1954 and Singh and Chaudhary

More about Opium Poppy

Open Document