One progress on TLC called high performance TLC (HPTLC; Sherma and Jain, 2000).HPTLC makes use of gel qualities that are finer, so that thinner plates and smaller. This allows faster separation times and better separation efficiency. HPTLC has improved reduced resolution and detection limits, so that the to walk two dimensions. To phospholipids visible on the TLC plates are used detection reagents. spots corresponding phospholipids may be carbonized by the addition of phosphomolybdic acid, sulfuric acid or copper sulfate in phosphoric acid, and then heating of the sample.
An example of use is cleaning compounds of impurities, where different solvents work as a molecular filter that interacts differentially with compound and impurities respectively. In the end of the process, impurities become physically separated from the now pure compound, facilitating further transferring and further repurification / extraction if needed. MATERIALS AND METHODS Acetanilide tert-butyl methyl ether p-tert-butylphenol p-toluic acid 0.5M NaHCO3 3M HCL 0.5M NaOH NaSO4 anhydrous Separatory Funnel Buchner filter paper Sand Bath Microspatula Disposable Pipet heated plate boiling chips support stand Pasteur pipets with latex bulb test tubes (2) utility clamp watch glasses weighing paper melting pint capillary tubes thermometer EXTRACTION •Dissolve acetanilide, p-toluic acid, and p-tert-butylphenol in t-butylmethyl ether •Extract p-toluic acid from the ether layer with NaHCO3 solution •Extract p-tert-butylphenol from the ether layer with NaOH
This is because Bentonite clay has adsorbing abilities due to its molecular shape. It has a large area that allows other molecules to adhere to it by the process of intercalation. A way to test this theory is with spectrophotometry. By obtaining the absorption spectrum of a red dye containing PAHs, one can compare that absorption to the absorption of the dye after Bentonite clay has been used to remove PAHs. Materials and Methods: To begin the experiment, the Bentonite clay had to be made.
The used acetate solutions are mixed with phosphate buffer which is followed by mixing with chloroform solution, than again with phosphate buffer and then with ether solution. Acidity should be neutralized to prevent any further hydrolysis. Penicillin in ether solution is mixed with with sodium bicarbonate. As a result of this mixing penicillin-sodium salt is obtained that can be stored as a stable powder at room temperature. The penicillin-sodium salt is obtained from the liquid material by basket centrifugation, in which solids are easily removed.
Decant(ing): Decanting is a way of separating a solid from a liquid. We can carefully pour a liquid from a solid, provided the solid is quite dense and resides on the bottom of a container. The easiest way to accomplish this is to hold a glass stir rod against the lip of the container containing the solid and liquid, and gently pour the liquid down the stir rod into a new container. Extraction: Extraction is a way to separate two components with different solubilities, that is, extract one chemical from another. Extraction relies on a principle called serial dilution.
A binding material can be separated by dissolving the tablet in ether since three active ingredients are soluble. Then, aqueous K2HPO4 and KOH are used to extract the ether solution with the conjugate base of the Aspirin layer and the conjugate base of the Acetaminophen, respectively. The caffeine can be isolated from the left over layer by evaporating the ether. Two separated solutions form solid by acidifying with HCl and the solid residue can be collected by vacuum filtration. According to the Revell's lab experiment, aspirin, acetaminophen, caffeine recoveries are 60% or higher, <10% and <5%, respectively.
Simple and Fractional Distillation of Cyclohexane and Toluene Purpose: The purpose of this experiment was to become familiar with the processes of simple and fractional distillation. In this experiment a mixture of two volatile compounds, cyclohexane and toluene, were separated with the process of distillation. Distillation relies on each compound having distinct and separate boiling points. The pure products were analyzed with gas chromatography to determine the success of the distillation. Introduction: Distillation is the process of heating a liquid until it boils, then condensing and collecting the resulting hot vapors.
This was so that the mussel samples would not go bad due to heat or harsh solvents. They were extracted with a mild solvent, and then concentrated by evaporation. The team found that the vapor was not toxic, but the residue after was. Other extractions were done to see how the residue separated when added with other mixtures of nonpolar solvents. In the toxic mussels, the visible light absorption spectrum revealed a pattern that was characteristic of phytoplankton pigments.
The Agilent 7890A Gas Chromatography System separates chemicals based on the ease with which they evaporate into a gas, also known as the volatility. The chemical mixture being analyzed is injected and carried through the instrument by an inert gas. There is a column in the oven that makes it possible to separate the chemicals based on how quickly they
2.3.2.2 Chemical Interesterification Chemical interesterification is the process to redistribute the component of fatty acid and glycerol ions to form new gylcerolester with the presence of sodium methoxide as a catalyst. Chemical interesterification is also known as directed interesterification since this reaction is directed to produce a particular type of glyceride (O’Brien, 2009). This process is conducted at mild temperature with a range between 20°C to 100°C. O’Brien (2009) states that only catalyst that is active at low temperature is effective. Besides, he also states that the rate of random rearrangement is crucial since the trisaturated glycerides can precipitate as fast as they are produced in the liquid phase.