Methionine Synthase Case Study

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Aim: The aim of the study is to evaluate Methionine Synthase (MTR) gene polymorphism in persons who are exposed to trace quantities of anaesthetic gases in operation theatres. Materials and Methods: 87 cases of physical status American society of anaesthesiologists (ASA) I and II exposed to anaesthetic gases in operation theaters at Gandhi Hospital & Osmania General Hospital, Hyderabad were selected for study. Also 150 controls who were not exposed to anaesthetic gases were selected at random to compare with the data generated on the subjects exposed to these gases. Results: This study entitled study of methionine “synthase (mtr) gene polymorphisms in personnel exposed to trace quantities of anaesthetic gases in operation theatres” was conducted…show more content…
The frequency of males was (34.5%) and females was (65.5%) in the exposed group while in nonexposed group the frequencies were 60% among males and 40% females. About 58.6% of the subjects had 1-10 yrs, 28.7% had 11-20yrs, 11.5% had 21-30yrs while only 1.2% had longer exposure of 31-40yrs to nitrous oxide. The mean BMI recorded for the subjects exposed to anaesthesia gases and controls was 24.0± 0.25 and 24.14±0.39 respectively. Table 2 shows genotype frequencies of c.2756A>G polymorphism of MTR gene. CASES CONTROLS Genotype N Frequency N Frequency A/A 39 51% 55 44% A/G 35 46% 64 51% G/G 2 3% 7 6% Table 3 shows Allele frequencies of c.2756A>G polymorphism of MTR gene. CASES CONTROLS Allele N Frequency N…show more content…
The allele frequencies were consistent with HWE in subjects exposed to anaesthesia gases but not in controls (AN=0.13; CT=0.059; Table-3). The deviation from HWE may be attributed due to genetic drift, migration, selection, etc. Table 4 shows risk of c.2756A>G genotypes under different models. Model Genotype Cases Controls OR (95% CI) P-value Codominant A/A 39 (51.3%) 55 (43.6%) 1.00 0.57 A/G 35 (46%) 64 (50.8%) 1.17 (0.64-2.12) G/G 2 (2.6%) 7 (5.6%) 2.26 (0.43-11.86) Dominant A/A 39 (51.3%) 55 (43.6%) 1.00 0.5 A/G-G/G 37 (48.7%) 71 (56.4%) 1.22 (0.68-2.21) Recessive A/A-A/G 74 (97.4%) 119 (94.4%) 1.00 0.35 G/G 2 (2.6%) 7 (5.6%) 2.09 (0.41-10.69) Overdominant A/A-G/G 41 (54%) 62 (49.2%) 1.00 0.76 A/G 35 (46%) 64 (50.8%) 1.09 (0.61-1.97) Log-additive --- --- --- 1.27 (0.76-2.12) 0.36 F Cases Controls OR (95% CI) P.VALUE A/A 27 (52.9%) 28 (51.8%) 1 A/G 23 (45.09%) 23(42.5%) 0.96 (0.44-2.11) G/G 1 (1.90%) 3(5%) 2.89 (0.28-29.56) 0.93 0.68 A/A 12(48%) 27(37.5%) 1 A/G 12(48%) 41(56.9%) 1.52 (0.60-3.87) G/G 1(4%) 4(5%) 1.78 (0.18-17.63) 0.38

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