Measuring Protein Concentrations in Unknown Solutions

645 Words3 Pages
Measuring Protein Concentrations In UNKNOWN Solutions Authors: Chelbi Copes Abstract: In this experiment, the concentrations of 7 different tubes of protein were determined, one of them being an unknown. The concentrations were determined in a spectrophotometer. It measures the amount of light that can pass through it and determines the concentration. The concentration of the unknown resulted as .233µg/mL. Introduction: This experiment was executed to determine the concentration 7 different solutions with different concentrations. The solution was part water and part Bovine Serum Albumin (BSA). To determine the concentration, a spectrophotometer was used. The use for a spectrophotometer is to determine the concentration of a liquid using light to determine the absorbency. Materials & Methods: First .5 mls of 2000 µg/ml BSA Standard was obtained in a test tube. Then a transfer pipette, 7 plastic tubes were filled with .25 ml of distilled water. Then using a pipette, .25 mls of the BSA from tube 1 and added it to tube 2. Then from tube 2, .25 mls of the solution was added to tube 3. Then from tube 3, .25 mls of the solution was added to tube 4. From tube 4, .25 mls of solution was added to tube 5. From tube 5, .25 mls of the solution was added to tube 6. From tube 6, .25 mls of solution was added to tube 7. The tubes were labeled from 0 to 7, tube 7 being the unknown concentrated solution. Another set of 7 tubes were obtained and 2mls of the BCA Kit Working Reagent (WR) were added to them. The WR was made by adding 50 parts of reagent A to 1 part reagent B supplied in the kit. The WR tubes were blue-green in color. Using a transfer pipette, 2 drops of protein solution was added to the corresponding WR tubes. Then the tubes were put in a 37 degree water bath for 25 to 30 minutes. After the tubes came out of the water bath they were all different shades of

More about Measuring Protein Concentrations in Unknown Solutions

Open Document