Then both test tubes were placed in a room temperature bath. Then 3-4 drops of iodine were added and adjusted. After 5 minutes, the position of the dye was recorded and then after every 2 minutes for 20 minutes the dye position was recorded. Time (minutes) Dye Position Test Tube #1 pt 30OC Test Tube #2 pt 100OC 0 0.1 0.1 2 0.1 0.1 4 0.1 0.1 6 0.11 0.1 8 0.11 0.1 10 0.12 0.1 12 0.12 0.1 14 0.13 0.1 16 0.14 0.1 18 0.14 0.1 20 0.15 0.11 Figure 1: Each test tube contained 20 pea seeds which were pretreated for 15 minutes in 0.2M sucrose solution at 30OC and 100OC. Each test tube contained a cotton ball dampened with 15% KOH, then a ball of glass wool, then 20 pea seeds soaked at the certain temperature, then another ball of glass wool, and then another cotton ball dampened with 15% KOH.
2 mL of a saturated calcium acetate solution was added to an evaporating dish. 15 mL of ethanol was added to the dish and swirled. The dish was ignited. 8. 3g of NH4Cl and 7g of Sr (OH) 2 * 8H2O were added to a 125 Erlenmeyer flask and swirled vigorously for 5 minutes.
The cations used were K+, Fe3+, Zn2+, Cu2, and Co2+. The first elimination test was metal hydroxide test where all five cations are used. 10 drops of each cation are placed in a centrifuge tub. 6M NaOH was added to each tube dropwise until a precipitate was formed. One cation is to be eliminated.
Predicting an Unknown from Logic Trees Project 2 Report Ashley Garcia* Ayland Smith Introduction The purpose of the project was to use elimination and confirmation tests on several anions and cations to form a logic tree. Cations used in part 1 are potassium, iron (III), zinc (II), copper (II) and cobalt (II), and the anions in part 2 are chloride, carbonate, nitrate, and sulfate. The unknown contains both a cation and an anion. The logic trees from part 1 and part 2 were then used to identify an unknown. Materials and Methods Part 1 For the cation elimination test first 10 drops of potassium, iron (III), zinc (II), copper (II), and cobalt (II) were added to 5 centrifuge tubes and the color was recorded.
Materials & Methods Identifying the unknown compound solution (#2314) first required the identification of each individual solution. One drop of each solution was added to five individual wells of a spot plate. Next, three drops of 3M hydrochloric acid (to be referred to from now on as HCl). The spot plate was then cleaned, and the procedure above was recreated two more times, the second test done by adding 3M ammonium hydroxide (NH4OH), and the third by adding 1 drop of dimethylglyoxime (DMG). All color observations were recorded and compared to the known behaviors of the focus metals.
3. Make three sets of the six test tubes and place a couple in the three different beakers 4. Place test tube 1 and 2 in the beaker that has a temperature of 0-10degrees. 5. Place test tube 3 and 4 in the beaker that has a temperature of 35-40degrees.
Confirmation tests are then used to confirm the found identity of the cation or anion. Materials and Methods: Cation Tests- The elements, potassium, iron (III), zinc, copper(II), and cobalt were each labeled on five test tubes, each consisting ten drops of the labeled metal solution. The color was noted. For the metal hydroxide elimination test, 6 M NaOH was added dropwise to each test tube till a precipitate was observed or till 20 drops were reached. 10 drops of NaOH were added to each precipitate to test for amphoteric species.
Labeled three test tubes 1, 2, and 3, and added 2 mL of milk to each. 5. Added 5 mL of Benedict’s Reagent to RS+, RS-, and tube #1, and boiled the tubes for three minutes. 6. Added one dropper full of Iodine to S+, S-, and tube #2, and agitated the tubes.
The milk was taken out from the water, then 15~20 drops glacial acetic acid were added. The milk was stirred with a stirring rod (every five drops were observed) until a white solid has formed. 5. Put cheesecloth on the top of 50 mL beaker, also using a rubberband to tie it. Separating the liquid and solid when the mixture was poured
After allowing the solution to sit for a few minutes, the liquid portion was separated into a waste container using a pipet. Once 10 mL of distilled water was added, 9 mL of 3 M H2SO4 was added dropwise until a color changed occurred. Then, 5 mL of distilled water was added to the remaining mixture and