Then, 3 drops of 3 M HCl was added to each of the solutions and the color of each was recorded. Then, the spot plate’s contents were put into the waste beaker and the plate was cleaned. After that, 1 drop of each of the five transition metal cations was added to separate holes in the spot plate, 3 drops of 3 M NH4OH was added to each hole and the color of each was recorded. Then the spot plate was cleaned again and 1 drop of each of the five transition metal cations was added to the separate holes. After that, 1 drops of dimethylglyoxime (DMG) was added to each and the color of each was recorded.
Introduction: This experiment was executed to determine the concentration 7 different solutions with different concentrations. The solution was part water and part Bovine Serum Albumin (BSA). To determine the concentration, a spectrophotometer was used. The use for a spectrophotometer is to determine the concentration of a liquid using light to determine the absorbency. Materials & Methods: First .5 mls of 2000 µg/ml BSA Standard was obtained in a test tube.
Record all data in a table and graph the results with the Volume on the x-axis, and the Mass on the y-axis. Part B: Mass and Volume of a Non-Cylindrical Metal 1.) Obtain a 25mL graduated cylinder. Fill it with 10mL of deionized water and record the volume in the nearest 0.1mL for the initial reading. 2.)
Stir each of the systems by drumming with your fingers on the loosely held test tube. To a 6th test tube, add about 1-ml Silver Nitrate solution. Label each test tube with the name of the chemical you used. Record these observations: System 1: FeCL3 System 2: KSCN System 3: K4Fe(CN)6 System 4: AgNO3 7. Mix pairs of solutions in the following ways, and record all of your observations in the data table.
The Vernier pH probe was calibrated and used to determine the pH of the phosphate buffer solution which was 6.81. Collectively 15.00mL of the slightly green solution was collected using a volumetric pipet and 5.00ml was transferred into 3 sterile 100ml beakers. A 1.0mL volumetric pipet was then rinsed in 1.0M HCL solution, the same pipet was then used to transfer 1.00mL of 1.0M HCL to one of the 3 beakers containing 5.00ml of the phosphate buffer solution stated previously. This beaker is now labeled Yellow. The same process is done with 1.0M of NaOH solution and the beaker was then named Blue.
The pH and temperature probe were used to record the pH and temperature of each of these three solutions. After obtaining four cuvettes, one was filled with distilled water, and the other three were each filled with one of the three solutions prepared earlier. First, the spectrophotometer was adjusted to 0%. Then the blank was inserted, and the reading was set to 100%. The transmittance % was then determined for each of the three colors at 360 nm.
These solutions should be in dropper bottles. Also concentrated HCl was be needed. The equipment need for this part of the experiment are 10 centrifuge tubes, a test tube rack, a distilled water bottle, medicine dropper, cobalt glass, Nichrome wire loop, striker, a 50 mL beaker, a stir rod, pipets, and a Bunsen burner. To begin the experiment, 5 centrifuge tubes were labeled for each of the 5 cations, K+, Fe3+, Zn2+, Cu2+ and Co2+. 10 drops of each solution was added to the appropriate centrifuge tube.
This report includes measurements collected from graduated cylinders, balances, and metric rulers, and the densities the data helped calculate. Hypothesis Based on our study of density, we should be able to calculate the mass and volume of each of the seven objects to find their densities and then determine what each of the objects are using the calculated density. Materials and Methods At Lab Station 1, a dry 100mL graduated cylinder was placed on a balance and the mass was recorded in grams to the nearest .01 gram. Approximately 60mL of the Clear Liquid 1 was carefully poured into the graduated cylinder. The graduated cylinder was placed on the scale again (with the added liquid) and the mass was measured in grams to the nearest .01 gram.
Repeat steps one and two 2. Place one drop of red dye into the test tube 3. Place one drop of yellow dye into the tube 4. Compare the controlled color to the new color 5. Clean up lab Data: Trial | # of Dye Drops | Color | 1 | 2 red, 1 yellow | Very dark red | 2 | 1 red, 1 yellow | Light colored red | Analysis: By first placing two drops of red dye, and one drop of yellow dye into the 10ml of water, the color became too dark of a red to match the controlled substance.
This experiment had two main phases, identification of transition metals and paper chromatography. The first step that was done was to create two sheets of chromatography paper that resembles the diagram inside the lab manual with six columns labeling solution 1-5 and the unknown solution. After this step was completed for both sheets, using a capillary tube for each transition metal, solution was added on each sheet in its respected place. The sheets were folded and stapled so that they were cylindrical with the writing on the outside. Next, each cylinder was placed in a separate 600mL beaker, one with about a centimeter of 6M HCl and the other with the same amount of 1.5M HCl.