Write down everything you notice. Remove test tube from water and allow it to cool for 2 minutes in a small beaker of tap water. Add about 7.0 mL of 1M hydrochloric acid to the tube. Mix chemicals very thoroughly with a stirring rod. Record how long it takes for change to happen.
The last two cations should be separated with the observations of the additional NH4OH added. With that all the cations are separated, a confirmation test was given to all the cations. 20 drops of each cation solution were placed in new centrifuge tubes. A Bunsen burner was set up with a sharp blue cone that was inside of the flame, then a Nichrome loop was dipped into HCl then dipped into one of the cation
Next, obtain a 5 mL serological pipet and thoroughly rinse it with the buffer solution, then discard the buffer solution into the 250 mL beaker. Now, use the pipet to distribute 5 mL of the buffer solution into three 50 mL beakers. Be sure that the 50 mL beakers have been cleaned are dried prior to this. Next, locate the three pre filled burets in the lab room. Find the buret labeled 1.0M HCl and add exactly 1.00 mL of HCl to just one of the three 50 mL beakers with buffer solution already in them.
Next was the ammonia test 10 drops of each metal solution were added to new centrifuge tubes and 15 M NH4OH was added until the solution changed color or a precipitate was formed. 10 additional drops of 15 M NH4OH were added and changes were recorded. For the confirmation flame test 20 drops of the metal solutions were added to centrifuge tubes. A Bunsen burner was set up using a striker to lite the flame, and a Nichrome loop was dipped in 10 mL of HCL and placed in the flame till no color was observed. Then the Nichrome loop was dipped in each tube of metal solution, (dipping the loop into HCL and in the flame after each solution) and placed in the flame and color was observed for each metal solution.
Then wash the zinc in the boiling tube “R” by adding 5 mL of acidified water, swirling and then decanting the solution into the “p” boiling tube. Repeat it 2 more times. | The liquid in the boiling tube “R” first starts out clear, and then once we add the acetic acid and swirl, the solution turns a yellow color, to then a brown-red color. The boiling tube
Then wash the zinc in the boiling tube “R” by adding 5 mL of acidified water, swirling and then decanting the solution into the “p” boiling tube. Repeat it 2 more times. | The liquid in the boiling tube “R” first starts out clear, and then once we add the acetic acid and swirl, the solution turns a yellow color, to then a brown-red color. The boiling tube
Remove the test tube from the hot water bath. Turn off the burner. Cool the test tube and its contents for 2 minutes in room temperature water. Add 1.5 M hydrochloric acid to the third mark. Mix with the stirring rod.
Repeat this two more times 5. Use a pipet to put 10 ml of the appropriate solutions in each bag: Bag 1: 10 ml – 10% sucrose Bag 2: 10 ml – Distilled Water Bag 3: 10 ml – 10% Sucrose 6. Squeeze as much air as possible out of the tubes and tie the remanding open sides shut. 7. Make sure to cut the excess string hanging off of each bag.
The contents were disposed of and rinsed with distilled water. For the flame confirmation tests, 20 drops of each metal solution were added to their appropriate tubes. A Bunsen burner was lit and adjusted till a blue cone appeared in the flame. A platinum loop dipped in HCl was placed above the
Allow the precipitate to settle, and heat 30 mL distilled water. Weigh, fold and place two filter papers into funnels and seal with distilled water. Decant a 50 mL sample from each beaker of solution into two other beakers. Pour the rest of the solutions into the respective funnel filters, and make sure to wash all precipitate into the filter, using the heated distilled water. Remove and air dry the filter papers containing the precipitates.