• Second, you close one end of the tube with a clamp. • Third, you place 10 ml. of solution A,B,C into separate dialysis bags with a pipet. (Solution A- with sucrose, solution B with twice as much sucrose, and solution C containing water.) • Fourth, clamp the other end of tubing, leaving about a 2 cm.
Observe the color change. Osmosis: The materials needed for the osmosis experiment include: 1%, 20%, and 40% sucrose solutions, a 100ml beaker, pipettes, 3 dialysis bags/tubing, scales, and weigh boats. The procedure was a follows; Place the 1% sucrose solution into a beaker. Next, fill one dialysis bag with 1% sucrose
The 15 M NH4OH was added drop wise until a color change occurred, or until 20 drops were added. An additional 10 drops of 15 M NH4OH were then added to each solution. Again, 5 centrifuge tubes were labeled for the same 5 cations and 20 drops of each solution were added to the appropriate centrifuge. HCl was also added to a 50 mL beaker. The Nichrome wire loop was dipped in the HCl solution and placed over the Bunsen burner to disinfect it.
The pH recorded by using pH electrode before adding NaOH. The solution titrated by 2 ml of NaOH each time and the pH recorded until the color change. These steps were done for the two known solutions and the unknown solution. Part 2 10 ml of vinegar placed in 100ml volumetric flask and deionized water added until the mark. The solution transferred in 150 ml beaker labeled as beaker #1.
Repeat steps 6-8 with the remaining chemicals, including the unknown. 10. All chemicals can be rinsed down the sink. Extinguish the flame by carefully replacing the cap over the flame. Data Table: | Sodium Nitrate | Barium Nitrate | Calcium Nitrate | Cupric Nitrate | Lithium Nitrate | Potassium Nitrate | Strontium Nitrate | Unknown | Flame Color | | | | | | | | | Results and Discussion: 1.
Then, using the pipette fill drop one drop of dish soap into both cups to act as a surfactant or “wetting agent”. Next we have to draw the gases out of the spongy mesophyll tissue and infiltrate the leaves with the bicarbonate solution. To do this, open your syringe and place ten of the leaf disks in one syringe and ten in the other. Once that is complete, take the syringes and fill one with 5cc’s of bicarbonate solution and fill the other syringe with 5cc’s of water (make sure all leaves are suspended and aren’t already floating. Now we have to create a vacuum in the syringe to draw the air out of the leaves.
Apparatus: • Safety glasses • Bunsen burner • Heat proof mat • Sodium thiosulphate • Hydrochloric acid • 100 ml of conical flask • Stop watch • 50 ml measuring cylinder • 10 ml measuring cylinder • Distilled water • Ice • Apron • Beaker Method 1. Collect safety equipment (apron and safety glasses) 2. Collect all materials and apparatuses 3. Set up the practical for room temperature test. 4.
* Pour a little ether over the nutmeg residue on the filter paper so that any Diethyl ethanol traces clinging to it is washed down and mixed with the filtered liquid underneath. * Filter the mixture by gravity filtration, washing the nutmeg residue with 10ml of diethyl ether. Evaporate the Ether from the filtrate * Recrystallize the product from ethanol. Filter using a Buchner funnel and wash them with cold water as shown in the diagram (see figure 2). * Let the crystals dry for one week, record the weight and take a sample and put into a glass capillary tube to obtain a melting point using the Melt-Temp machine.
In the back lab, we mixed the urea water in a big jug and took all the urea water for the conicals out of the jug so we knew that the concentration of urea would be consistent. The dyes we chose were black, bright orange, blue, emerald green, and banana. Next, we measured out how much dye we would need to make the 200%, 100%, 75%, 50%, 25%, and 0% solutions using 50 mL of urea water per each conical. So since we used five different dyes and six different concentrations we required 30 conicals. Once all the dyes were separated by concentration
Composition of Agar jelly. Temperature of the room. Apparatus: 1) Agar jelly 2) HCl – 1 molar 3) Petri dish 4) Cutting board 5) Scalpel 6) Stop watch 7) Pipette Procedure: Cut the agar into different sizes of blocks with a knife and measure the size with the help of a ruler to make an accurate experiment. Put some hydrochloric acid into a petri dish and make sure that it will completely cover an agar block. Then place the agar into the test tube including hydrochloric acid, start timing immediately by the help of a stopwatch.