ENZYME worksheet 1. Label the diagram [pic] a. substrate b. active site c. enzyme d. products 2. Answer true of false to the following statements: ___T____ Enzymes interact with specific substrates ___F____ Enzymes change shape after a reaction occurs ___T____ Enzymes speed up reactions. ___F____ One enzyme can be used for many different types of chemical reactions. ___T____ Enzyme reactions can be slowed or halted using inhibitors.
Explain what it means to denature an enzyme. -To denature an enzyme means to change its shape by breaking the hydrogen bonds that holds its shape, thus rendering it useless. An enzyme can be denatured with heat, or with a change in pH. 5. What factors did you need to control in your tests?
DETERMINING THE PROPERTIES OF AN ENZYME I. Abstract Enzymes are responsible for the speed at which chemical reactions they are involved in take place. This experiment determines the effects that concentration, temperature, pH, and boiling have on an enzyme’s ability to perform its work. It is hypothesized that none of these variables will have any effect on the activity of enzymes and these hypotheses are tested using dye-coupled reactions to determine the rate at which peroxidase converts H2O2 into water (H2O) and oxygen (O2). Each hypothesis is subsequently rejected as data suggests that concentration, temperature, pH, and boiling all have an effect on enzyme activity. II.
Hereditary Fructose Intolerance and Mitochondrial Disease Sara James Western Governor's University Hereditary Fructose Intolerance Enzymes in Breakdown of Fructose Enzymes are proteins that carry out chemical reactions. They will bind to a substrate and then end up releasing a product. The enzymes do it by a process of lock and key. The lock is considered the substrate and the enzyme is considered the key. Only the enzyme will fix the substrate because of the active sites on the enzyme.
They are used to speed up the reaction rates by providing an alternative reaction pathway of lower activation energy, which is the minimum energy that requires for reactions occur. Catalysts take part in a reaction (chemical involved), but they do not undergo any changes in chemical reaction. (Farabee, 2001) Enzymes are proteins made of a combination of different amino acids. Therefore, the enzymes may be lost
INTRODUCTION Enzymes are a type of protein that has a key role in helping cells carry out particular chemical reactions. It is a protein that is capable of speeding up chemical reactions by lowering the energy required to activate the reaction. It remains unaltered in the process and can be reused. Substrates, or reactants, are the original molecules before a chemical reaction occurs. The molecules that are the result of the chemical reaction is called the product.
Denatured sucrase was used as a control because denatured enzymes, in this case sucrase, lose their shape and can potentially lose their ability to catalyze a chemical reaction. Using deantured sucrase as a control helps to further solidify and/or compare findings from solutions
Explain why it is important to denature the enzyme. It is important to determine if ph, temperature and sucrose do have a true effect on active sucrase. leaving it active would increase the activity of the enzyme
If a simple chip has the power to mimic the functions of the human body, then it is a huge resource to research. Animals are not needed for testing with such a wide range of alternatives. These new methods stretch each dollar spent on experimentation because the cost of the replicas and chip are only a fraction of the cost of animal experimentation. Alternatives provide a chance for medical
Therefore, they prevent the enzyme reactions from taking place. Independent Variable: Presence or absence of metal ions in the solution There are two solutions with two different metal ions and two are replaced by distilled water without metal ions Dependent Variable: The effect of metal ions on the enzymes activity of intervase It can be measured via Benedict’s test by the colour change and the quantity of the precipitate formed Controlled Variable: The amount of the invertase in each solution The time of the reaction take place Control set-up: Replace the metal ions in the solution by the same volume of distilled water Assumptions: All reducing sugar has reacted with B solution, invertase works well in the given pH and temperature Materials and apparatus: * 10mM lead (II) nitrate * 10mM silver nitrate * 50g/L sucrose solution * 0.03g/L invertase solution * Benedict’s solution * 6 test tubes * Measuring cylinder * 1 beaker * Bunsen Burner * Tripod * Wire gauze Procedures: Silver nitrate: 1. 0.5mL of silver nitrate solution is added to 1mL of sucrose solution. 2. 1.5mL of invertase solution is added to the mixture.