DNA Review Questions 1. Examples of polymers that contain repeating units known as nucleotides are: a. Hemoglobin b. Starch c. Cellulose d. DNA e. Paints 2. How many different bases are associated with the makeup of DNA? a. Two b.
Replication Fork In the DNA double helix Topolisomerase relieves the tension. When Helicase breaks down the hydrogen bonds replication begins. Replication can take place in 2 directions because of the replication bubble. The enzyme Primase synthesizes the RNA primers. There has to be primers to start the synthesis at the 3’ end of the new strands.
The first step of DNA replication is the unwinding of the two individual strands of DNA that are together in a structure that is known as a “double helix”, a term coined by Watson and Crick, who founded the first original model of DNA. The enzyme that is used to split the two strands is called helicase, and the splitting process starts in a place called the “origin of replication”. After each separate DNA strand has successfully unwound, the bases that are present on the strands are now exposed, and unpaired. The enzymes then match the bases with the free nucleotide triphosphates. The bases used in DNA replication are adenine (A), thymine (T), guanine (G), and cytosine (C).
Transgenesis and Cloning Transgenesis is the process of inserting a gene from one source into a living organism that would not normally contain the inserted gene. The gene can come from the same species (called Cisgenesis) or from a different species entirely. To facilitate the transfer of genes from one organism to another, often a Transgenic Organism with Recombinant DNA is created: -The first step in creating an organism capable of carrying out the transformation process is to isolate the required gene. This is done so using Restriction Enzymes, which target a specific gene sequence. The gene is often cut with staggered ends, called “Sticky Ends” which only allow specific and complementary gene sequences bond by base pairing.
Describe each process (including differences between bacteria and eukaryotes) and explain the significance of the differences between replication and transcription When first going through DNA replication, the two strands of double helix unwind. Each strand is an outline for the formation of a new, complementary strand. DNA helicase enzymes hang along the DNA molecule, opening the double helix as they move. Once the strands are separated, helix-destabilizing proteins bind to single DNA strands, preventing re-formation of the double helix until the strands are copied. Enzymes called topoisomerases produce breaks in the DNA molecules and then reconnect the strands, relieving strain and effectively preventing tangling and knotting during replication.
Conclusion 1. Explain the relationship between the following words – cells, genes, chromosomes, tissues, DNA, proteins. * * Tissues are made of cells that contain chromosomes made of DNA with regions called genes that code for proteins * 2. Explain why scientists probably used PCR when they prepared the DNA you’re your experiment. * * PCR is the process of copying DNA.
Each genome contains the information needed to maintain and create the organism. The process of genetic engineering involves extracting of a small piece of cellular DNA, called a plasmid, from the bacteria if organism involved in the manipulation. A very small section of the circular plasmid is then cut out by the restriction enzymes which act as molecular scissors. The gene from the organism being modified is then inserted into this space and the plasmid is therefore modified. The genetically modified plasmid is now inserted and introduces into a new organism which starts divides rapidly.
Basically, DNA controls protein synthesis. The complex and precise process of protein synthesis begins within a gene, which is a distinct portion of a cell's DNA. DNA is a nucleic acid which is made up of repeating monomers, called nucleotides, and in the case of DNA, these individual monomers consist of a pentose sugar, a phosphoric acid and four bases known as adenine, guanine, cytosine and thymine. DNA is a double stranded polymer, which has a twisted ladder like
Running head: DNA AND THE DOUBLE HELIX DESIGN DNA And The Double Helix Design J. Schultz University of Phoenix DNA And The Double Helix Design DNA, the commonly used acronym for deoxyribonucleic acid, is the carrier of the genetic code for most living organisms. DNA contains the genetic codes that determine eye color, hair color, and sometimes diseases. Each trait that passes from parent to offspring is the result of information carried by DNA. Possible Reasons For The Double Helix Shape DNA has a double helix shape that is functional and useful in several ways. According to The National Center For Biotechnology Information (2004): The chemical nature of the bases in double-stranded DNA creates a slight twisting force that gives DNA its characteristic gently coiled structure, known as the double helix.
Remember: *DNA must be fully condensed in order to divide. * M Phase is made up of mitosis and cytokinesis. The mitosis part has five phases: 1) prophase 2) prometaphase 3) metaphase 4) anaphase 5) telophase Following telophase is cytokinesis: division of the cytoplasm, organelles and macromolecules. Prophase -DNA is completely condensed. We can see it…this means there are condensin proteins.