Discovering the Effects of Temperature Changes on the Tonoplasts and Plasma Membranes of Red Beet Root by Examining the Amount of Betacyanin Released from the Cell’s Vacuole

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Linea Eby January 31, 2011 Hao Xu Discovering the effects of Temperature Changes on the Tonoplasts and Plasma Membranes of Red Beet Root by Examining the Amount of Betacyanin Released from the Cell’s Vacuole Introduction: The semi-permeable plasma membrane of the cell serves many functions. It regulates the transportation of macromolecules in and out of the cell, holds the cell together, and protects the cell from its external environment (Biology 107 Lab Manual, 2011). When environmental conditions are altered outside the cell, the membrane shields the cells organelles from some of the damage. If environmental conditions are so great that they begin to damage the shielding properties of the membrane, the organelles will be subjected to the full extent of the detrimental conditions. Organelle damage can have a variety of consequences. In this experiment we will be specifically looking at the effects of a variety of temperatures on the vacuole of red beet roots. The beet cells large central vacuole (where Betacyanin is found) is separated from the cell’s cytoplasm by a membrane known as the tonoplast (Biology 107 Lab Manual, 2011). The composition of this membrane is much like the plasma membrane that surrounds the cell. Like the plasma membrane, the tonoplast is made up of a phospholipid bilayer with embedded proteins and steroids (Lin et al., 2008). This composition is known by biologists as the fluid mosaic model (Campbell Biology, 2011). Due to the close relation between these membranes, factors causing the deterioration of one membrane will likely affect the other equally. The plasma membrane, being more external, will receive the brunt of the impact but once it is degraded the tonoplast will likely suffer similar damage. If the tonoplast and plasma membrane are damaged the contents of the vacuole (Betacyanin in particular) will be released from the

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