Results and Discussion For the first part of the experiment (Part A), five different 100 mL volumetric flasks were each filled with 1,2,3,4 and 5 mL of iron (II) solution. Then 5 mL of YY ligand, were poured to each of the five flasks. Each flask had 5 mL of 2M sodium acetate and 4 mL of 3M NH2OH. Then the whole solution was diluted up to the 100 mL fill mark with distilled water. This was the solution that was used in order to obtain the absorption spectrum for each of the different iron (II) ligand examples different flasks.
5mL of acidified water will be measured, using a graduated cylinder, and will be transferred to the R tube, and will be immediately vigrously mixed with the reactants. Once the solution turns to an orange or red-brown color, a pipet will be used to quickly remove 30 drops of the solution, then transferred to the C tube, and the mixing will resume until the solution is close to room temperature. The solution will be filtered into the P tube, and the solution that is left in the R tube should be washed three times with 1mL of acidified water each time. The water should then be poured into the P tube, leaving the solid in the R tube. Using a test tube holder, heat the R tube over the Bunsen burner, moving the tube in a circular motion until all the water has evaporated.
c. Prepare the solution by dissolving 38.90 grams of ZnI2 with 500 mL of water. d. 0.0125/0.25 = 0.05 L = 50 mL. This produces 0.0125 moles of ZnI2 5. Exercise 5: a. (0.125)(0.1) = 0.0125 moles of solute b. Pour 50 mL of the stock solution to get the number of moles needed.
Twenty drops of bromothymol blue was also added to the 150mL beaker. The pH was then obtained using the Vernier pH probe and it read 6.68. The 5mL pipet was then used to transfer 5mL of the green solution to the three 50 mL beakers. A 100mL beaker was obtained and filled with 1.0 M HCl solution and the pipet was used to acquire 1mL of the 1.0 M HCl solution. The 1mL of HCl was then transferred to one of the 50mL beakers turning the color of the solution to yellow.
Then 5mL of HCl was added to copper to completely remove all traces of zinc. Once the bubbling had stopped, the rest of the liquid was decanted away from the copper. Then the copper recovery set up was put together using tubing, Buchner funnel, filter paper and suction flask. Then the filter paper was weighed before placing it in the funnel and wetted down. The aspirator was turned to medium high, and then the copper was poured onto wetted filter paper.
Obtain beaker filled with 250 mL of HCl and 5 pennies. 2. Get a pipette and remove 10 mL of that solution (HCl + Zn) and place it in a 100 mL beaker. 3. Add color indicator 4.
Add a quarter spatula of copper (II) oxide and warm the solution gently to the 4th test tube and record observations. 7. To the 5th test tube, add 3cm3 of ethanol a couple of drops of conc, sulphuric acid and warm gently. Pour the resulting mixture into 30cm3 of sodium carbonate solution to remove excess acid and smell and record observations. Experiment 2 Time | Observations | 5 minutes | Bubbled like sugar | Once salt water was added | Turned soapy white and thick | Equation: METHOD 1) Put 2 cm3 of castor oil into a 250 cm3 beaker and add 10 cm3 of 5mol.dm-3 sodium hydroxide from a measuring cylinder.
Gather all materials 2. Heat 200mL of water in the beaker for 90 seconds 3. Place the bulb of one thermometer just below the surface of the water 4. Record the initial temperature at the top 5. At the same time, place the second thermometer bulb just at about the bottom of the beaker 6.
About 50 mg of the powdered mycelium was transferred into a microtube contained 500 µl of TES (100 mMTris, pH 8.0, 10 mM EDTA, 2% SDS). To which, 50 µg proteinase K was added and incubated for 1 h at 60°C with occasional gentle mixing. To the above mixture, 140 µl of 5 M NaCl was added to adjust the salt concentration to 1.4 M. Then 65 µl of 10% CTAB (CetylTrimethyl ammonium bromide) was added and incubated for 10 min at 65°C. To the above mixture, 700 µl of Chloroform and isoamyl alcohol (24:1) was added, mixed gently, incubated for 30 min at 0°C and centrifuged at 10000 rpm for 10 min at 4°C. The supernatant was transferred to a 1.5 ml tube; to which 225 µl of 5 M NH4Ac was added, mixed gently, incubated on ice for 30 min and centrifuged at 10,000 rpm for 15 min at 4°C.
Put exactly 5.0 mL of water in the 10.0 mL graduated cylinder. Record this volume in your data table (10.0 mL). Label the first pipet "Acid." To calibrate the pipet, fill it with LIU-2 water. Holding the pipet vertically, add 20 drops of water to the cylinder.