Chromatography Lab Report

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Paper Chromatography Chemistry Lab Report 1 (2015) Final Version Name: Harry He Class: AP Chem 3 Date: Sep.17 , 2015 Terminology Affinity – natural attraction or force between things. Capillary Action – the movement of liquid within the spaces of a porous material due to the forces of adhesion, cohesion, and surface tension. Chromatogram – the graphic record produced by chromatography. \ Mixture – a material composed of two or more elements or parts. Mobile Medium – gas or liquid that carries the components (moile phase). Solubility – the quality, condition, or degree of being soluble or solvable. Solution – a mixture of a solvent and a solute. Solvent – a substance that dissolves…show more content…
The two figures shows the relative affinity of components in the chromatograph and the distance components travel compares to the distance water travels. #1 True colour Green contained 2 dyes: Bright Blue and Yellow. The Affinity of Blue dye was 3.1/3.5 = 0.94[4] while the affinity of yellow dye was 0.7/3.5 = 0.28. Thus the relative rate of migration of bright yellow is ; the relative rate of migration of bright blue is . #2 Miff Green contained 2 dyes: Blue and Yellow. The Affinity of Blue dye was 0.97 while the yellow dye didn’t travel. #3 True colour Purple contained 2 dyes: Purple and Light Blue. The Affinity of purple dye was 0.84 while the blue dye was 0.12. #4 The components of Miff Purple were unable to be separated in this experiment. The components remained unknown in this experiment. #5 The components of Marvel Green were unable to be separated in this experiment. The components remained unknown in this…show more content…
Oil or Alcoholic base is more effective in Chromatography for separation that could identify the components in Marvel Green. Potential Improvement Chromatography Roll[8] If you have numerous samples that need to be put into one solution and you are using a large beaker, then you can put the samples on one piece of filter paper instead of several strips. Follow the original process but use a big paper instead Roll the big strip and staple the edges (make sure the edges do not touch) Conclusion Although the colour contains similar dyes in colour, they are different substances. In this experiment, many dyes are not separated because of the inefficacy of the H2O base. To analyse the component more deeply and comprehensively, we should conduct in different bases such as CCl4 and C2H5OH. Reference of the Lab Report Nanjing University textbook, Common Chemistry (Chinese Version, translated by the author) Texas A&M University, Protein Chemistry, Laboratory Lab report written by Sabrina Schmidtke Whitham, Christopher. “Lab 1: Paper Chromatography” Class Handout for Chemistry, 2015 The formula created by Mихаил Семёнович Цвет Chemistry, September (analytical chemistry)1996 (China Chemistry

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