Do an initial Benedict's test on the 15% glucose/1% starch and the beaker solutions for glucose by putting some of the solution and a roughly equal amount of blue Benedict's solution in a test tube, placing the test tube in boiling water for 90 seconds, and observing whether or not the solution changes color from blue. 3. Form a bag out of dialysis tubing by tying off one end, putting in enough 15% glucose/1% starch solution to fill it halfway, and tying off the other end leaving the other half of the bag void of anything (even air). Write down the solution's color. 4.
First I made a water bath by filling the 100 mL beaker with cool tap water. I then placed crushed ice in the 100 mL beaker so the water level was just below the top of the beaker. I sprinkled a little salt in the ice water and mixed it well. I then filled the test tube half full with distilled water and set the test tube in the 24 well plate. I inserted the digital thermometer into the test tube and took reading every 30 seconds until the readings remained constant.
Glucose levels were measured at 0.52, 0.57, and 0.67 showing that an increase in surface area corresponded with a non-significant (p=0.096) increase in the glucose diffusion rate. Introduction Diffusion is the random movement of substances from an area of higher concentration to an area of lower concentration (down a concentration gradient) and this occurs to stabilize the cell (Kaisa, 2011). Diffusion occurs until the particles are completely evenly distributed. Several factors can alter the diffusion rate of substances, but specifically several factors can influence the diffusion rate of glucose from potatoes. Changes in temperature, surface area, soaking time of the samples, and many other factors may influence the diffusion rate of glucose.
Materials: 3 beakers Thermometer 3 Alka-Seltzer tablets Stopwatch Mortar and pestle Source of hot water Ice cubes Graph paper Procedures: Hot water- Run water from the hot tap until it is hot as possible Fill beaker with 80 mL of hot water Use thermometer to take temperature of water. Record in data table. Remove 1 Alka-Seltzer tablet from package Drop into the water. Measure the time it takes for the tablet to completely dissolve. BE READY WITH THE STOPWATCH.
Less germinated in the presence of 1% ammonium nitrate compared to the spores not in the presence of ammonium nitrate. In other studies done, when ammonium is used to provide the nitrogen source for photosynthetic spores, they will not grow or germinate properly, and may show abnormalities and inhibited growth (Melan & Whittier, 1990). That is exactly what our experimental data showed us. After preforming the tests and compiling all the replicate data, we found that in the control group there was an average of 50.5 germinated spores per plate. In the plates containing 1% ammonium nitrate there was an average germination rate of 28.5 spores per plate.
Writing Assignment 4 Name: Michael Tran FlyGene Unknown Code: 230 PS ID #:1109766 Lab Section: 16241 BIOL 3311 - Spring 2012 TA Instructor Name: Sneha Koneru Date: March 29, 2012 Materials and Methods In order to contain and cultivate Drosophila melanogaster flies, 25 x 95 mm polypropylene vials are used and are enclosed with foam stoppers. Provisions are made from a dehydrated cornmeal-based formula called 4-24 Blue and is prepared by combination with diluted water. The appropriate ratio of fly food is obtained by measuring half of the provided metal food cup with 4-24 Blue and mixing it with 7 ml of diluted water using a 15 ml conical test tube. Once complete, four granules of dry baker’s yeast are placed on the hydrated food as a nutritional supplement and to prevent the growth of bacteria. To manipulate the flies, they are anesthetized using CO2 that is administered through the FlowBuddyTM device with a 5 L/min flow rate.
10 mL of each solution [0.16M KI, 0.0055M (NH4)S2O8, 0.12M Na2S2O3, and water] were added to an Erlenmeyer flask along with about 0.2 g of starch and a drop of EDTA (to prevent coagulation) and mixed with a stir bar. The reaction was conducted twice for room temperature (24.5ºC), cold (1.5ºC), and warm (37.0ºC). Observations were made as the mixture changed from clear to a dark blue, almost black. The time for this color shift was recorded (in seconds). By varying the temperatures, variables A and Ea could be determined.
Measure 50.0 mL of 2.0 M Sodium Hydroxide, NaOH, solution but DO NOT ADD YET. Click the Collect button to get the initial temperature of the HCl solution. After the first four readings have been recorded at the same temperature, add the NaOH solution. Make sure you have lowered the paper lid to keep from liquid coming out of the cup. Turn the Stirrer on, at a slow to medium speed.
Cook for 4 more minutes, then drain liquid from pot and pour contents over a clean, unused large garbage bag or newspapers. Serve with melted butter and enjoy!! Let the water boil in a pot that is 3/4 full with two seasoning packets and 2-4 cut up lemons. Add 2 bottles of Newman’s
To grow the E. coli, 190ml of 2% glucose nutrient media was added to a 250nl Erlenmeyer flask. 10ml of nutrient media was used to blank the spectrophotometer set at OD600. 10ml of E. coli stock culture were added to the 190ml nutrient media, creating a 1:20 dilution. The inoculated nutrient media was then placed into a 37C orbital shatter to incubate. All solutions measured with the spectrophotometer were placed in spectrophotometer tubes.