Antifungal Bacteria for Sugarcane Disease

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Epicoccum nigrum P16, a Sugarcane Endophyte, Produces Antifungal Compounds and Induces Root Growth ´ ´ ´ Leia Cecilia de Lima Favaro1*, Fernanda Luiza de Souza Sebastianes2, Welington Luiz Araujo3 ´ 1 Brazilian Agricultural Research Corporation, Embrapa Agroenergia, Brasılia, Distrito Federal, Brazil, 2 Department of Genetics, ‘‘Luiz de Queiroz’’ College of Agriculture, ˜ ˜ ˜ ˜ ˜ University of Sao Paulo, Piracicaba, Sao Paulo, Brazil, 3 Department of Microbiology, Biomedical Sciences Institute, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil Abstract Background: Sugarcane is one of the most important crops in Brazil, mainly because of its use in biofuel production. Recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potential. Epicoccum nigrum is an important sugarcane endophytic fungus that has been associated with the biological control of phytopathogens, and the production of secondary metabolites. In spite of several studies carried out to define the better conditions to use E. nigrum in different crops, little is known about the establishment of an endophytic interaction, and its potential effects on plant physiology. Methodology/Principal Findings: We report an approach based on inoculation followed by re-isolation, molecular monitoring, microscopic analysis, plant growth responses to fungal colonization, and antimicrobial activity tests to study the basic aspects of the E. nigrum endophytic interaction with sugarcane, and the effects of colonization on plant physiology. The results indicate that E. nigrum was capable of increasing the root system biomass and producing compounds that inhibit the in vitro growth of sugarcane pathogens Fusarium verticillioides, Colletotrichum falcatum, Ceratocystis paradoxa, and Xanthomomas albilineans. In

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