Biology Lab Report Photosynthesis

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An investigation of the light-dependent electron transport using DCPIP Introduction Photosynthesis is a reduction-oxidation reaction, which uses carbon dioxide, water and light to produce water and sugar. During this reaction water is oxidised and carbon dioxide is reduced (Sadava et al. 2011). There are two parts to photosynthesis, the light-dependent reaction and light-independent reaction. The light-dependent reaction consists of changing light energy to chemical energy for the formation of ATP and NADPH. The light-independent reaction use carbon dioxide and the products of ATP and NADPH from the light-dependent reaction to form glucose (Sadava et al. 2011). The purpose of this experiment is to determine the various ways the rate of photosynthesis is affected by the chloroplast under different treatments. The indictor DCPIP can determine the rate of photosynthesis. DCPIP is able to capture the electrons that are transferred through the electron transport chain will cause the intensity of the indicator to decrease. The decrease of the indicator associates to an increased rate of photosynthetic activity (Miyazaki et al. 2012). It was hypothesised that the isolated chloroplasts from silverbeet leaves exposed to while light (Tube 3); the tube wrapped in red cellophane (Tube 6) would have a faster rate of photosynthesis, than the chloroplast kept in the dark (Tube 2), the boiled chloroplast suspension (Tube 4), the addition of DCMU (Tube 5) and the tube wrapped in green cellophane (Tube 7). Methods Table 1. Experimental design for the electron transport experiment. | | | | | | TREATMENT | | | | | BLANK 1 | DARK 2 | LIGHT 3 | BOILED 4 | DCMU 5 | RED 6 | GREEN 7 | A | chloroplast suspension (ml) | 1.5 | 1.5 | 1.5 | - | 1.5 | 1.5 | 1.5 | B | buffered sucrose (ml) | 5.5 | 5.3 | 5.3 | 5.3 | 5.2

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