Hypothesis: If bacteria with + pGLO plasmids that are resistant to the antibiotic ampicillin and have the gene for GFP, colonies with survive and grow on the transformation plates that have LB/ amp. In addition, + pGLO bacteria on a plate with LB/amp/ara will grow and glow green under UV light because of the inclusion of arabinose. In the control plates, -pGLO bacteria that are amp sensitive will not be able to grow on the LB/ amp plates. The other control plates with – pGLO bacteria and no ampicillin added will host a lawn of colonies. IV.
Purpose In this lab we are trying to get a broader understanding of the transformation of bacteria by exposing them to pBLU plasmids. Introduction Transformation is the manipulation of a bacterial cell's DNA in order to alter the cell's genotype or phenotype by absorbing free DNA from its surroundings. This can result in a nonpathogenic bacteria becoming pathogenic by absorbing the DNA of a broken open or dead pathogenic bacteria. In our case it is taking in the pBLU plasmid. A plasmid is a spherical self-replicating DNA molecule that is not actually a part of the bacterial cell but can integrate itself into the bacterial chromosome.
1-2-09 Glowing Bacteria Genes are pieces of DNA which hold the instructions for making proteins. The protein will then give an organism a particular trait. Genetic transformation means changes caused by genes, and requires the insertion of a gene into an organism, in order to change the trait. this technique is used by in many areas of biotechnology. Agriculturally, plants can be genetically transformed to be resistant to pesticides, spoilage, and frost.
By using the genes from two bacteria producing organisms such as Alcaligenes Eutrophus, they could insert them into cress plants with the aim of these plants producing the PHB. This process worked and the plants and some of their offspring produced around 14% of the dry weight as PHB. Although this is not as high yielding as when it is produced in the petri dishes, it is a much more economically viable method of production and hence would give a much more substantial impact to society. Chemically PHB is from a group of biopolymers known as the polyhyroxyalkanoates. Figure 1 below shows other examples of biopolymers in
The sexually transmissible disease gonorrhea has become increasingly resistant to treatment with antibiotics. What is the most likely scientific explanation? a) The gonorrhea bacteria learned to avoid antibiotics b) The gonorrhea bacteria changed their genes when they sensed antibiotics c) Antibiotic-sensitive gonorrhea bacteria died out, but antibiotic-resistant gonorrhea bacteria have flourished and persisted d) The antibiotic increased the mutation rate in the gonorrhea bacteria e) Both b and d 26. Which statement best describes the relationship between plants and animals on earth? a) Plants produce O2 and sugars from CO2 b) Animals produce CO2 and H2O from sugars and O2 c) Plants produce O2 and sugars and animals produce CO2 and H2O d) Animals produce O2 and sugars and plants produce CO2 and H2 27.
4. Yeasts are growing in two dishes. You treat one with a chemical that blocks DNA replication but forget to label it. How can you identify the treated dish? One dish will have more visible DNA then the other one, which will show which one was used by the chemical and which dish was not
The first one is called somatic-cell gene therapy. This kind is aimed at introducing genes into some of the somatic cells to correct a genetic defect. It is limited to the person involved and will not be transferred to future generations. The second one is Gem-line gene therapy, which would result in the correction of the genetic problem in an individual’s reproductive cells so that it would no longer be passed on to his or her offspring. The third one is referred to as Enhancement genetic engineering.
It is obvious that when people pay money for something they buy, they want to know what exactly things are (such as nutrition percentage or calories content). The foods we eat are particularly related to people’s health. Second, genetically modified food can unexpectedly transform some components into different ones and cause allergies in some people. To illustrate, genes that were changed in genetically modified foods may contain strange substances that produce allergic reactions for purchasers. For example, tomatoes are injected with fish gene to make a new product with better quality because the components in fish can protect fruit from freezing.
Pros and Cons of Genetic Engineering While genetic engineering can lead to introduction of greater quality traits in organisms, it can also have undesirable side effects. To understand the pros and cons of genetic engineering, read on. The science of indirectly manipulating an organism's genes using techniques like molecular cloning and transformation to alter the structure and nature of genes is called genetic engineering. Genetic engineering can bring about a great amount of transformation in the characteristics of an organism by the manipulation of DNA, which is like the code inscribed in every cell determining how it functions. Like any other science, genetic engineering also has pros and cons.
These genes carry the instructions to build a new person; they are made up of a substance called deoxyribonucleic acid, commonly known as DNA. DNA produces proteins that regulate the development of an individual human being. A Good example of how the genes interact and the environment would be the genetic disorder called Phenylketonuria, commonly known as PKU for short, this is a rare genetic condition that prevents a person from being able to process a number of food. PKU prevents an individual from processing a substance called phenylalanine, this is found in many foods. This condition causes a build up of a number of harmful substances in the body that in turn damages the brain and can eventually kill the individual.