The supernatant was transferred to a 1.5 ml tube; to which 225 µl of 5 M NH4Ac was added, mixed gently, incubated on ice for 30 min and centrifuged at 10,000 rpm for 15 min at 4°C. Then the supernatant was transferred to a fresh tube, 510 µl of isopropanol was added to precipitate the DNA and centrifuged immediately for 10 min at 10,000 rpm. The supernatant was removed; the pellet was washed twice with cold 70% ethanol, air-dried and suspended in 50 µl TE
Procedure: 1. Fill a beaker two-thirds full of water and add approximately 20 drops of IKI. Write down the solution's color and record the mass of the bag. 2. Do an initial Benedict's test on the 15% glucose/1% starch and the beaker solutions for glucose by putting some of the solution and a roughly equal amount of blue Benedict's solution in a test tube, placing the test tube in boiling water for 90 seconds, and observing whether or not the solution changes color from blue.
Juliana Park Mayumi Tamada CHEM 111B LAB/ M-F 1-4PM 15 August 2012 Spectroscopy Lab Introduction In this lab, the molar absorptivity of the complex FeLn2+ will be determined by using the absorbance of the complex and its concentration. The absorbance will be found by using a spectrophotometer. For the next part of the lab, the formula of the complex will be determined by also using the volume of ligand and the absorbance again. Experimental There are two different parts to the experiement. In the first part, five 100 mL flasks of 5 mL ligand solution, 5 mL 2 M sodium acetate, 4 mL 3 M NH2OH, and 1-5 mL Fe2+ solution are diluted with water.
The following laboratory results were obtained: CHEMISTRY PANEL REFERENCE RANGE Na 128 mmol/L (136-146 mmol/L) K 5.7 mmol/L (3.5-5.0 mmol/L) Cl 88 mmol/L (98-109 mmol/L) HCO3 9mmol/L (22-28 mmol/L) BUN 50 mg/dl (5-20 mg/dl) Osmolality 310 mOsm/kg (285-295 mOsm/kg) pH 7.12 (7.35-7.45) pCO2 28 mmHg (35-46 mm Hg) Glucose 750 mg/dl (70-105 mg/dl) Urine Glucose 4+ Serum Acetone 3+ 1. Identify all abnormal values. Indicate if the value is increased or decreased when compared to the reference range 2. On the basis of the patients clinical history, laboratory data and clinical findings, what type of glucose intolerance would be the most probable? Explain how the history, data and findings helped you identify the type of glucose intolerance exhibited by the patient.
The liquid of homogenate was filtered into a beaker through Miracloth (2 layers cloth) to remove large plant components and 1 ml of the filtrate was transferred to a conical tube. 8.4 g of ammonium sulfate was slowly added to the 40 ml of the filtrate as it was stirred on a stir plate for 15 min to achieve 37% saturation (210g/L of solution). The solution was then centrifuged at a speed of 9000 x g at 4oC for 15 min to sediment the proteins. The resultant supernatant 1 was transferred to a beaker with 1 ml transferred to a conical tube and the obtained pellet 1 was resuspended in 4 ml of distilled water and transferred into a dialysis bag to remove the salt. Then, 3.4 g of ammonium sulfate was slowly added to the supernatant 1 as it was stirred for 15 min to achieve 50% saturation (85g/L of solution).
Calculate the molarity of the original vinegar solution and its concentration in gdm-3, given that it reacts with NaOH in a 1:1 ratio. 7. 2.5 g of a sample of ethanedioic acid, H2C2O4.nH2O, was dissolved in water and the solution made up to 250 cm3. This solution was placed in a burette and 15.8 cm3 were required to neutralise 25 cm3 of 0.1 moldm-3 NaOH. Given that ethanedioic acid reacts with NaOH
Again the test tubes were placed in a spectrometer and their absorbance recorded every twenty seconds for a full two minutes. Results: Enzyme Activity Time (sec) Tube 2 & 3 .5mL Extract Tube 4 & 5 1.0mL Extract Tube 6 & 7 2.0mL Extract 20 .11 .01 1.4 40 .11 .01 1.9 60 .11 .01 2 80
The purity of each opiate was ~98% by HPLC peak and normalization method. The stock solution of each estimated compound was prepared separately. The quantification was performed using linear regression curve. Validation: TLC-densitometric procedure was applied to quantify the five major opium alkaloids morphine, codeine, thebaine, papaverine and narcotine (Gupta and Verma, 1996). Toluene-acetone-methanol-ammonia (40:40:6:2) v/v was used as a mobile phase.
Mirco-Scale Acid-Catalysed Iodination of Acetone. Abstract: The purpose of this experiment was to titrate different concentrations of HCl and ClCHCOOH against Na2S2O3. The volumes found were plotted against time and values for Kobs , KH+ and KHA were found from these graphs. The Kobs values found for each experiment were (i)Kobs = 2.48x10-6, (ii)Kobs = 6.2x10-7, (iii)Kobs = 5.3x10-7, (iv)Kobs = 4.6x10-7. The values found for KH+ for the reaction using HCl as the acid were found to be (i)KH+ = 4.96x10-5, (ii)KH+ = 1.24x10-5, (iv)KH+ = 1.84x10-5.
Micro – scale Reduction of a Ketone to an Alcohol: Benzophenone to Diphenylmethanol with Sodium Borohydride Abstract: Purpose of experiment 2.1 was to perform reduction reaction of benzophenone to diphenylmethanol with sodium borohydride as a reducing agent. The reducing agent was used in excess to ensure complete reduction of the carbonyl group. The product was isolated as a solid by filtration and its purity was checked using Thin Layer Chromatography with different ratios of mixture of polar and non – polar solvents and by checking its melting point, which was 520C - 620C. Infra – Red spectroscopy was not performed, however previously printed graphs were compared and analysed based on tables in Chemistry Laboratory Manual. Purpose of the Experiment: To produce diphenylmethanol from the reduction of benzophenone by using sodium borohydride as a reducing agent.