Mol Biotechnol (2007) 37:169–180 DOI 10.1007/s12033-007-0031-3
History of plant tissue culture
Trevor A. Thorpe
Published online: 27 June 2007 Ó Humana Press Inc. 2007
Abstract Plant tissue culture, or the aseptic culture of cells, tissues, organs, and their components under deﬁned physical and chemical conditions in vitro, is an important tool in both basic and applied studies as well as in commercial application. It owes its origin to the ideas of the German scientist, Haberlandt, at the begining of the 20th century. The early studies led to root cultures, embryo cultures, and the ﬁrst true callus/tissue cultures. The period between the 1940s and the 1960s was marked by the development of new techniques and the improvement of those that were already in use. It was the availability of these techniques that led to the application of tissue culture to ﬁve broad areas, namely, cell behavior (including cytology, nutrition, metabolism, morphogenesis, embryogenesis, and pathology), plant modiﬁcation and improvement, pathogenfree plants and germplasm storage, clonal propagation, and product (mainly secondary metabolite) formation, starting in the mid-1960s. The 1990s saw continued expansion in the application of the in vitro technologies to an increasing number of plant species. Cell cultures have remained an important tool in the study of basic areas of plant biology and biochemistry and have assumed major signiﬁcance in studies in molecular biology and agricultural biotechnology. The historical development of these in vitro technologies and their applications are the focus of this chapter. Keywords Cell behavior Á Cell suspensions Á Clonal propagation Á Organogenesis Á Plantlet regeneration Á Plant transformation Á Protoplasts Á Somatic embryogenesis Á Vector-dependent/independent gene transfer
T. A. Thorpe (&) Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4 e-mail: firstname.lastname@example.org
Introduction Plant tissue...