Extraction and Distillation

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Objective/Purpose The objective of a distillation process is to demonstrate methods for purification of volatile compounds (compounds that can be converted to gas phase at reasonable temperatures without decomposition) and to demonstrate that gas chromatography may be used to quantitatively determine the relative proportions of volatile compounds in each sample. Using three different separating and analyzing methods, we will be able to study what compounds were in our tomato paste. Materials - A glass distillation set up (including all of the little pieces) - Heating mantle - Thermostat - 100 mL round bottom flask - Beakers - Graduated cylinder - Stirring rod - TLC silica gel plates - Jar - Dichloromethane - 50/50 % hexane and acetone - Scale for weights - 7:1 hexane and dichloromethane mix for TLC - Tomato paste - Funnel Filter - Wool - Heating mantle Methods Weigh out approximately 10.00 grams of tomato paste. Then, retrieve 50/50% hexane/acetone. Next, get 2.00 grams of MgSO4 and combine it with the paste. Place pieces of wool into the neck of a funnel and place the funnel over a beaker. Mix 15 mL of 50/50% into the beaker, stirring to get a liquid from the paste. Then, pour just the liquid into the funnel to filter it out. Do this one more time the exact same, and then a third time using only 10 mL of the mixture. Each of the three times you do this, extract the liquid. Transfer the liquid into the large 100 mL round bottomed flask that is connected to your simple distillation set up. Distill the tomato paste liquid. Once it is completely distilled, remove your filtered material and add 2 mL of dichloromethane. Swish the flask, and then place into a small beaker. Next, place the beaker with the distilled liquid on a heating mantle and heat to a gel like substance. Make sure not to burn it. The next processes that will be
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