Determination Of Caffeine In Tea By Hplc

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QUANTIFICATION OF CAFFEINE IN TEA BY HPLC Learning Outcomes On completing this experiment you should be able to: * Prepare a series a series of calibration standards. * Extract an analyte from a sample matrix. * Quantify the concentration of the analyte. * Estimate the uncertainty of your analysis. * Describe the operating procedures for batch samples on a HPLC instrument. Important Information * This experiment carries 6.25 % of your marks for the module. * The experiment will be performed with a partner but you should submit separate and individually written reports. * The deadline for submission is 23:59 hrs on the Tuesday after your experiment. No marks will be given for late submission. * All submissions will be checked for plagiarism and copying. Uploading Your Reports * Meet the deadline. * Your report must be submitted as a Microsoft Word document (.doc or .docx) or as a .pdf file * It is recommended that you adopt the following file name convention: “Caffeine in Tea report < First name Family name> <Student No.>”.doc/.docx/.pdf e.g. Caffeine in Tea report Richard Ellis A9500111.doc * Upload your report to the 10CMB015 module page on Learn Safety Information The samples and solvents used in this experiment pose little hazard provided that routine laboratory precautions are taken to avoid ingestion or skin contact. Care is required when handling the warm water for the extraction. Minimise the length of the time the samples are carried and held. If the warm water does come in contact with the skin, rinse with cold water and seek first aid attention. Seek guidance from a technician or demonstrator before using the HPLC instrument. Note the detector contains a UV source which should not be viewed directly. Preparation Organise yourselves to work well as a team,

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