The cell wall of the bacteria 3. What is iodine used for in this staining technique? A. Iodine is used to affix the primary stain crystal violet to make a large molecule that will not be easy to be washed away. 4. Why is it important to heat fix
The concentration of a solution is the amount of solute that is dissolved into each unit of solvent. In this lab, water (H2O) will be the solvent and sugar/sucrose (C12H22O11). When the density of the solute is greater than the density of the solvent, the density is directly proportional to the concentration of the solute. The relative density of the solute to the solvent can be observed by placing some solute into the solvent without mixing. If the solute floats, then the density of the solvent is greater than the density of the solute.
The second simulated cell will be 1% sucrose solution placed into a 50% sucrose solution. I believe this simulated cell will show us the hypertonic part of this lab. Because of the cell having a less concentration than the sucrose solution it is being place, water will move out of the simulated cell and into the surrounding solution trying to even out their environment. The third simulated cell will be 50% sucrose solution placed into a 1% sucrose solution. I believe this simulated cell will show us the hypotonic part of this lab.
This will occur because the water and glucose are small enough to diffuse out of the pores in the dialysis bag, but the starch molecules are too large to fit through 2. If a solution is very hypertonic, then the most amount of water will diffuse across the semi-permeable membrane into the dialysis tubing. This occurs because the higher concentration of solute, the more water it takes to reach equilibrium, thus, the faster rate of osmosis. 3. If we place the potato in a sucrose solution with a similar solute concentration as a potato, then the least amount of water will diffuse into or out of the potato cells.
Joel Barlow High School Honors Biology Michael Klein Wassink 11-5-2014 Lab: pH, Buffers & Biological Substances Problem How do organisms survive and function despite metabolic activities that tend to shift pH toward either acidic or basic ends of the scale. Hypothesis I predict that as I add varying amounts of 0.1m HCl and 0.1m NaOH to various biological substances the pH levels of those biological substances will go down with the addition of HCl and up with the addition of NaOH because Acids (HCl) release H+ into substances which makes their pH levels go down, and Bases (NaOH) accept H+ from substances which makes their pH levels go up. Materials • 4 goggles • 4 aprons • 50ml beaker • 1-100ml-grad cylinder • 3-colored pencils • Wide range pH paper • Tap water • Forceps • HCl (0.1M) • NaOH (0.1M) • pH 7 buffer solution • Milk • Potato homogenate • Egg white • Gelatin suspension Procedure 1. Pour 25ml of tap water into the 50ml beaker. 2.
Chemistry PAT Question 1 Polyhydroxybutyrate (PHB) is a biopolymer with properties very similar to that of polypropylene, though has all the benefits that come with biopolymers. PHB was first introduced to the scientific community by the scientist Maurice Lemoigne in 1926. He noticed that to produce PHB you need particular micro-organisms such as Alcaligenes Eutrophus or Bacilius megaterium undergoing physiological stress in an apparatus such as an agar dish to allow the micro-organisms to multiply. In order to achieve this stress there is a restriction to the amount of nitrogen the micro-organisms can attain thereby altering its nutrition. This is quite an efficient method of production and the amount of the PHB polymer yielded is around 30-80% of the dry weight of the micro-organisms used.
EXP 2.13: MEASUREMENT OF THE CRITICAL MICELLE CONCENTRATIONOF A SURFACTANT ABSTRACT The aim of this experiment is to measure the critical micelle concentration of sodium dodecysulphate. This will be done by using the conductivity method which will involve measuring the conductivity of a solution during a titration. The second part of the experiment will involve using spectrophotometric method to determine the maximum wavelengths at different concentrations. INTRODUCTION in colloidal and surface chemistry the critical micelle concentration (CMC) of a surfactant is the concentration where micelles begin to form [1]. Surfactants are compounds that contain hydrophobic and hydrophilic groups.
Lesson written by Carolina Sylvestri Experiment: Reaction Between Ions in Aqueous Solutions The Monster Mash Background: Ionic solids dissolve in water to form aqueous solutions which conduct electricity. These solutions contain both positive and negative ions in such numbers that their net electric charge is zero. In this experiment, you will mix various ionic solutions, two at a time, to determine which combinations form precipitates. Knowing which ions are present makes it possible to deduce which of the possible ion combinations are responsible for the precipitates. From your data table, it will then be possible to generate a solubility table.
Add a drop of water using and inoculating loop to a clean glass slide. Then using a sterilized loop, smear the slide with the organism, let the slide dry completely and heat fix. Apply crystal violet stain (primary stain) for one minute, briefly rinse with distilled water. Apply iodine (mordant) for one 1 minute, briefly rinse with distilled water. Then proceed to decolorize with 95% ethanol until the ethanol running off the slide is clear.
* What is this substance This substance is iodide Confidence Report The aim of extraction, is to separate a part of raw material, using a solvent such as water with heat. A tincture of iodine is a common medicine sold in many pharmacies, used as an antiseptic on cuts of the skin. The objective of the seaweed lab, is to produce a tincture of iodine by extracting Iodide and other components (seen when a mixture of iodine is present), from seaweed. We