Yeast Reproduction in Sugar Substitutes

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YEAST REPRODUCTION IN SUGAR SUBSTITUTES Johnna Ruth C. Lopez1 & Rose Tifanny A. Pol1 1Department of Biology, College of Science, Polytechnic University of the Philippines, Sta. Mesa, Manila ABSTRACT Yeast utilizes the glucose in its environment to make energy. Equal (aspartame) and Splenda (sucralose) are both man made sugar substitutes. These substances aren’t supposed to have any calorie content so yeast life shouldn’t be possible. 6 mL room temperature water, 0.6mL sugar and 0.6mL yeast were poured into each test tube. The tube was then shaken gently. The volume (mL) of the mixture was measured after 30 minutes. Five trials were done with each substance. The trials including sugar had an average volume of 7.4 mL. The trials using Splenda had an average volume of 5.2 mL. The trials with Equal had an average volume of 7.9 mL. Aspartame had showed the most growth. Splenda had some dextrose (sugar) in it, which might have been the reason for growth. A possible reason for the trials of aspartame to produce more growth is that aspartame really could be used by the yeast. Keywords: Yeast, Sucralose, Aspartame, Sucrose, Dextrose INTRODUCTION Yeasts are unicellular, aerobic or anaerobic organisms. Yeast, like animals, gives off CO2 as a byproduct of respiration. Yeasts reproduce through budding in which an adult yeast cell grows an offspring from its body. For reproducing yeast cells need energy. Yeast utilizes the glucose in its environment to make energy. Sugars are made of 5 or 6 carbon atoms, along with hydrogen and oxygen, and they are called monosaccharide. They can bond together to form disaccharides. Aspartame, saccharine, and Splenda (Sucralose) are all man made sugar substitutes. Aspartame is made of carbon, oxygen, nitrogen, and hydrogen; its structure is not similar to sugars. Saccharine consists of carbon chains, oxygen, hydrogen,
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